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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

Plasmid

#102245

Purpose

Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

Depositor

Jacob Corn

Insert

dCas9-VPR-mCherry

Use

CRISPR and Lentiviral

Tags

NLS-VPR-mCherry

Expression

Mammalian

Promoter

CAG

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pC017 - LwCas13a guide expression backbone with tRNAval promoter

Plasmid

#91907

Purpose

Backbone for cloning LwCas13a guides under tRNAval promoter

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LwCas13a Guide site

Expression

Mammalian

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pUbC-nls-ha-stdMCP-stdGFP

Plasmid

#98916

Purpose

synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

Depositor

Robert Singer

Publication

Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

Insert

stdMCP-stdGFP

Use

Lentiviral

Tags

GFP

Promoter

UbC

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV2.5-THP-GFP

Plasmid

#80336

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Publication

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMAZ-SK

Plasmid

#73962

Purpose

self-killing gRNA plasmid pMAZ-SK

Depositor

Alex Nielsen

Publication

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Insert

gRNA

Expression

Bacterial

Promoter

pTet

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EGFP-Actin-7

Plasmid

#56421

Purpose

Localization: Actin, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Publication

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Actin (ACTB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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pCS2-Cas9

Plasmid

#47322

Purpose

For in vivo expression of Cas9 or generation of Cas9 mRNA

Depositor

Alex Schier

Publication

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR; Xenopus and zebrafish expression

Expression

Mammalian

Promoter

SP6

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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AAV-EF1a-BbChT

Plasmid

#45186

Depositor

Dawen Cai, Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has service

AAV9

Insert

pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE

Use

AAV; Adeno-associated viral

Expression

Mammalian

Promoter

human EF1a

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET His6 LIC cloning vector (2Bc-T)

Plasmid

#37236

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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FUW-Sox10

Plasmid

#36978

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox10 (SOX10 Human)

Use

Lentiviral

Expression

Mammalian

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Publication

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMSCVpuro-Cre

Plasmid

#34564

Purpose

Retroviral expression of Cre

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLV.PGK.mFoxa2

Plasmid

#33014

Depositor

Malin Parmar

Publication

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Forkhead box A2 (Foxa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

H10L

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-CD68 promoter/enhancer

Plasmid

#34837

Depositor

Peter Murray

Publication

Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Insert

CD68 promoter/enhancer -680/+140 (CD68 Human)

Expression

Mammalian

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pGL3-mArg1 promoter/enhancer -31/-3810

Plasmid

#34571

Depositor

Peter Murray

Publication

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Snail_pGL2

Plasmid

#31694

Depositor

Paul Wade

Publication

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Snail (SNAI1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pLenti CMVtight Hygro DEST (w769-1)

Plasmid

#26433

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Expression

Mammalian

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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