We narrowed to 9,202 results for: sacs
-
-
pFA-LEU2-I-SceI
Plasmid#67595PurposeI-SceI expression regulated by tetracycline inducible system in S. pombeDepositorInsertsnmt terminator
I-SceI
TetR
UseGene targeting in yeastTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4-2T
Plasmid#28250DepositorInsertsingle chain Cse4-Scm3-H4 with 2 Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3. An…PromoterAvailable sinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AktAR
Plasmid#123356PurposeFRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.DepositorInsertLyn-AktAR
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ1808 pRS416-COF1RFP
Plasmid#37103DepositorInsertCOF1-RFP
UseTagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …PromoterAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYF-2xSYN-tTA
Plasmid#19982DepositorInsert2xSYN-tTA (SYN1 Budding Yeast, Human, E.coli)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCaSper4 cyto roGFP2-Orp1
Plasmid#64998PurposeExpression of cytosolic roGFP2-Orp1 under the alphaTub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH-macroHC
Plasmid#82337PurposeProvides a C-terminal macro domain and 6xHis tag for expression in E. coliDepositorInsertSPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
UseTags6xHis non-cleavable and macro domain of human his…ExpressionBacterialMutationTruncated protein, amino acids 1-178PromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioA
Plasmid#65347Purposesysthesis of vioA gene in the violacein pathwayDepositorInsertvioA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)
Plasmid#136544PurposeExpresses ANXA2 chimeras in bacterial cellsDepositorTypeEmpty backboneUseTagsANXA2 (29-339) and His-tagExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV(X) C/EBPbeta
Plasmid#13393DepositorInsertC/EBPbeta (Cebpb Rat)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
POM43-mCherry
Plasmid#69857PurposeN-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmCherry minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL7Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSV(X) C/EBPalpha
Plasmid#13392DepositorInsertC/EBPalpha (Cebpa Rat)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pB194
Plasmid#61154PurposeExpression of KIN28 under GAL1 promoter in yeastDepositorInsertTFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGal1Available sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85857PurposeSensor PlasmidDepositorInsertmiR-21-3p Sensor (MIR21 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
LDB204
Plasmid#185426PurposeBacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusionDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 FXFG-repeats domain expressedPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
-
pTH652-RLuc/maxFLuc
Plasmid#29698DepositorInsertFirefly Luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)
Plasmid#136546PurposeExpresses ANXA2 chimeras in bacterial cellsDepositorTypeEmpty backboneUseTagsANXA2 (23-339) and His-tagExpressionBacterialMutationPromoterT7Available sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_2pabPAC
Plasmid#158968Purpose2P-activatable bPAC for transient expressionDepositorInsert2pabPAC
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-CDS1-3HA
Plasmid#198596PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorInsertCDS1 (CDS1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-DGK1-3HA
Plasmid#198595PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorInsertDGK1 (DGK1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-PAH1-3HA
Plasmid#198597PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorInsertPAH1 (PAH1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABF2-2EGFP-ter-URA3
Plasmid#184768PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertABF2 (ABF2 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.HA.HA.Mm.RAP1
Plasmid#184642PurposeRetroviral expression of mouse RAP1DepositorInsertRAP1
UseRetroviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only