We narrowed to 4,307 results for: ARA-2

1. pET28b-hTIG3-1-132

   Plasmid

   #100720

   Purpose

   Expression of N-terminal region of human TIG3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   RARRES3 (PLAAT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-MNTS-1

   Plasmid

   #182944

   Purpose

   To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

   Insert

   MNTS-1 (NTF3 Human, Homo sapiens, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   This is the Human NT-3 with mutations on the matu…

   Promoter

   CMV promoter and enhancer

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-HypocratesCS

   Plasmid

   #161957

   Purpose

   Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

   Depositor

   Dmitry Bilan

   Article

   Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

   Insert

   HypocratesCS
   

   Use

   Tags

   6xHIs tag

   Expression

   Bacterial

   Mutation

   changed cysteine 355 to serine

   Promoter

   T5

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1 tTA UQCRC2

   Plasmid

   #177978

   Purpose

   All-in-one dox-repressible expression of cDNA

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   UQCRC2 (UQCRC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to UQCRC2 sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1 tTA SDHB

   Plasmid

   #177977

   Purpose

   All-in-one dox-repressible expression of cDNA

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to SDHB sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pClneoFLAG human Dendrin

   Plasmid

   #47934

   Purpose

   expresses FLAG tagged human Dendrin in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Kawata et al J Biochem. 2006 May;139(5):931-9.

   Insert

   Dendrin-1 (DDN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   contains amino acids 2-711 of human Dendrin

   Promoter

   CMV

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pLentiCRISPRv1_SDHB

   Plasmid

   #177981

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv1_SDHA

   Plasmid

   #177980

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHA (SDHA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+-HypocratesCS

   Plasmid

   #161960

   Purpose

   Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

   Depositor

   Dmitry Bilan

   Article

   Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

   Insert

   Hypocrates
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 355 to Serine

   Promoter

   CMV, SP6

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMonAID_nCas9-PmCDA_Hyg_ALS

    Plasmid

    #91693

    Purpose

    Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Expression

    Mutation

    D10A;H840A nuclease-deficient

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOD2045-intControl

    Plasmid

    #100005

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

    Plasmid

    #156442

    Purpose

    For transient expression of mouse PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse PDS5B (Pds5b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1P POLE1_2

    Plasmid

    #160764

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160742

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1P DDX11_2

    Plasmid

    #160778

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1P DDX11_1

    Plasmid

    #160777

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge