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pMG035 MBP-Axin∆BCD-His

Plasmid

#154053

Purpose

Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-1-170aa-YFP

Plasmid

#29596

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-170aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-170

Available Since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-413aa-YFP

Plasmid

#29599

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-413

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-101-526aa-YFP

Plasmid

#29603

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-101-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 101-526

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-453aa-YFP

Plasmid

#29600

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-453aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-453

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-267-526aa-YFP

Plasmid

#29605

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-267-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 267-526

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pTE3320

Plasmid

#107529

Purpose

Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hLbCpf1(RVR)

Tags

3xHA and NLS

Expression

Mammalian

Mutation

G532R, K538V, Y542R

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-285-526aa-YFP

Plasmid

#29606

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-285-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 285-526

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-270aa-YFP

Plasmid

#29597

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-270aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-270

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-368-526aa-YFP

Plasmid

#29607

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-368-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 368-526

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pVB240306

Plasmid

#250660

Purpose

E. coli expression of the GSM domain of mouse ChREBPalpha (aa 43-207)

Depositor

Charles Brenner

Article

Tiwari et al Nat Metab. 2025 Nov;7(11):2284-2299. doi

Insert

ChREBP alpha GSM domain (Mlxipl Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

contains aa 43-307 only, with optimized E. coli c…

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pFB-EF1a-DIO-Rpl22l1-myc-Flag-2A-tdTomato-WPRE-hGHpA

Plasmid

#246243

Purpose

Cre dependent flag-tagged Rpl22l1.

Depositor

D. James Surmeier

Article

Tanimura et al Neuron. 2019 Feb 6;101(3):444-458.e6. do

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

Myc-FLAG-T2A-tdTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS1

Plasmid

#239460

Purpose

For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci Adv. 2025 Aug 29;11(35):eadw9153. do

Type

Empty backbone

Tags

VENUS:3xHA:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS2

Plasmid

#239459

Purpose

Simultaneous monitoring of transcription and translation of genes in plant tissues: N- terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci Adv. 2025 Aug 29;11(35):eadw9153. do

Type

Empty backbone

Tags

3xHA:VENUS:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLOC-D133p53a

Plasmid

#241916

Purpose

Lentiviral vector driving a p53 isoform delta133p53alpha. Stable expression in cultured human cells and possibly cells of other species.

Depositor

Curtis Harris

Article

Ungerleider et al Neuroscience. 2022 Aug 21;498:190-202. d

Insert

Delta133p53alpha (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-mCherry-1x miR5 Mm ATP10B

Plasmid

#216391

Purpose

transfer plasmid for AAV vector production of mCherry and miR for Mm ATP10B

Depositor

Veerle Baekelandt

Article

Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

Insert

mcherry and miR Mm ATP10B

Use

AAV

Promoter

CMVenh synapsin

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-mCherry-1x miR7 Rn ATP10B

Plasmid

#216392

Purpose

transfer plasmid for AAV vector production of mCherry and miR for rat Rn ATP10B

Depositor

Veerle Baekelandt

Article

Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

Insert

mcherry and miR Rn ATP10B

Use

AAV

Promoter

CMVenh synapsin

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase

Plasmid

#223188

Purpose

Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

luciferase (LOC116160065 firefly)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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