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Vector 1174K

Plasmid

#221017

Purpose

Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv [Preprint]. 2024 Jul 18:2024.07.

Insert

Engineered dobulesex splicing module (Anopheles gambiae)

Use

Synthetic Biology

Expression

Insect

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIB1884-ximD-PCPT

Plasmid

#169109

Purpose

Production of marmesin in Streptomyces xiamenensis

Depositor

Minjuan Xu

Article

Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

Insert

XimD and PcPT

Use

Synthetic Biology

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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AlACR2_EYFP_pcDNA3.1

Plasmid

#161023

Purpose

Expresses AlACR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR2

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR3_EYFP_pcDNA3.1

Plasmid

#161024

Purpose

Expresses AlACR3 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR3

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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RsCCR2_EYFP_pcDNA3.1

Plasmid

#159125

Purpose

This plasmid encodes RsCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RsCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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RsCCR1_EYFP_pcDNA3.1

Plasmid

#159124

Purpose

This plasmid encodes RsCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RsCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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RaCCR2_EYFP_pcDNA3.1

Plasmid

#159123

Purpose

This plasmid encodes RaCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RGS8

Plasmid

#140585

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RGS8

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-GTPBP2

Plasmid

#140586

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA GTPBP2

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD113

Plasmid

#121912

Purpose

Charge-Introduced NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

S110K, I111K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD168

Plasmid

#121916

Purpose

Oth-NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

I111K, E116K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBHDuet50K

Plasmid

#118399

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Expression

Bacterial

Mutation

incorporation of oligonucleotide during cloning

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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NONO2_IDR-EGFP

Plasmid

#232758

Purpose

Expresses NONO2_IDR-EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

NONO2_IDR-EGFP (NONO Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

Plasmid

#216248

Purpose

HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human RANGAP1 (RANGAP1 Human)

Use

CRISPR and TALEN; Hdr template

Tags

miniIAA7.3xFlag.P2A.BSD

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NFIA+SOX9

Plasmid

#129455

Purpose

Inducible overexpression of NFIA and Sox9 in human cells.

Depositor

Su-Chun Zhang

Article

Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

Insert

Sox 9 (SOX9 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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