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pL1M-pMtRC-MtLAP1-Adh-R1

Plasmid

#170790

Purpose

A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

Depositor

Jeremy Murray

Publication

Ruan et al Plants (Basel). 2021 Mar 23;10(3). pii:

Insert

MtLAP1 (LOC11412013 Medicago truncatula)

Expression

Bacterial and Plant

Promoter

Medtr4g059670(MtRC)

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-1P-Kv-WPRE

Plasmid

#202612

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Publication

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLdCN2

Plasmid

#125186

Purpose

Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

Depositor

Greg Matlashewski

Publication

Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pK1E-RNAP

Plasmid

#48144

Purpose

Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

Depositor

Dieter Jahn

Publication

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

rna polymerase K1E

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pGMCKq1-10M1-sspBmyc

Plasmid

#27069

Depositor

Dirk Schnappinger

Publication

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

tetR-PmyctetO-sspB

Expression

Bacterial

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirAonly

Plasmid

#232584

Purpose

Express HA-BirA

Depositor

Sagar Bhogaraju

Publication

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BirA

Tags

HA

Expression

Mammalian

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVNP3.0-PEmax

Plasmid

#206883

Purpose

Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Publication

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Publication

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Plasmid

#135458

Purpose

Mammalian expression of SPARK TF component

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Use

Lentiviral

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-EYFP

Plasmid

#35519

Depositor

Karl Deisseroth

Publication

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PX459-TP53-exon4

Plasmid

#217455

Purpose

For TP53 knockout targeting exon 4 of TP53

Depositor

John Diffley

Publication

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-PQRv3-RFP

Plasmid

#73951

Purpose

Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

Depositor

Brian Chen

Publication

Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

sfGFP (superfolder GFP)
RFP

Tags

A residual PQR peptide wii be left at the C-termi…

Expression

Mammalian

Promoter

Chicken beta actin and Chicken beta actin (shared…

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only
GFP-pcDNA3-PKCgamma-cys1Acys1B

Plasmid

#21190

Depositor

Tobias Meyer

Publication

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKCgamma-cys1Acys1B (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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V1663 pLPS-3'RFP - Acceptor

Plasmid

#11701

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP

Expression

Mammalian

Available since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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U6-pegRNA-H1-nick sgRNA-mCherry

Plasmid

#176901

Purpose

Expresses prime editing guide RNA and guide RNA in mammalian cells

Depositor

Hyongbum Kim

Publication

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

mCherry
gRNA scaffold
gRNA scaffold

Use

AAV

Expression

Mammalian

Promoter

CAG, H1, and U6

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDA027

Plasmid

#131385

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Publication

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A, D702Y

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMAL-c5X-MAP

Plasmid

#66999

Purpose

Expresses malE-MAP in E. coli

Depositor

Ashutosh Chilkoti

Publication

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-MAP

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMU-TLEr

Plasmid

#61187

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Publication

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

C160S

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article