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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,800 results for: emb

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Showing: 3841 - 3860 of 11800 results

  1. mcherry-p62 delta PB1 silent

    Plasmid
    #187985
    Purpose
    human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552
    Depositor
    Sascha Martens
    Publication
    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p62 (NUP62 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Lacking PB1 domain (aa1-102) with a silent mutati…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187985-plasmid-map-sequence-id-374442

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  4. pEF NS34a-msGFP-A'Q-mito

    Plasmid
    #158763
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Publication
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NS3/4a protease and msGFP
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158763-plasmid-map-sequence-id-312944

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  5. pelB-MBP-P66-ss

    Plasmid
    #137045
    Purpose
    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66949.1 (p66 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P66: lacks the P66 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137045-plasmid-map-sequence-id-291951

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  10. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid
    #114143
    Purpose
    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Publication
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    Expression
    Mammalian
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    114143-plasmid-map-sequence-id-224089

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  14. FB030

    Plasmid
    #119714
    Purpose
    Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator paf
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119714-plasmid-map-sequence-id-237150

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  20. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

    Plasmid
    #175261
    Purpose
    labelling of microtubule plus end and F-actin structures
    Depositor
    Frank Bradke, Sebastián Dupraz
    Publication
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
    Tags
    mCherry (on iLifeact) and mNeonGreen (on EB3)
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pTub-alpha1
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    175261-plasmid-map-sequence-id-349034

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Showing: 3841 - 3860 of 11800 results