We narrowed to 13,996 results for: eng

Showing: 3841 - 3860 of 13996 results

Results per page:

Sort results by:

pCFJ2369 - intron GG1 - 250bp

Plasmid

#159877

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG1 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

Plasmid

#98975

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 4 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EKARdual

Plasmid

#99870

Purpose

Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

EKAR

Tags

ShadowG and mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-PA(WT)-Vav2

Plasmid

#86977

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVNP3.0-PEmaxRH

Plasmid

#206884

Purpose

Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEMax Delta RNAseH

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ284 (Mb2Cas12a)

Plasmid

#138116

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PX394 Neisseria cinerea Cas9

Plasmid

#68331

Purpose

Neisseria cinerea Cas9

Depositor

Feng Zhang

Insert

NcCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJY-RpABE-PDS3_IMS

Plasmid

#112875

Purpose

binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3

Use

CRISPR

Expression

Plant

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

Plasmid

#200277

Purpose

Characterisation of the sialic acid-responsive circuit with fluorescent reporter

Depositor

Matthew Chang

Article

Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

Inserts

CadC
NanR

Expression

Bacterial

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

Plasmid

#167901

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKD2-ChR2-mCherry

Plasmid

#170845

Purpose

AAV vector for Cre-dependent transgene expression of ChR2-mCherry in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Liu et al Elife. 2020 Oct 9;9. pii: 55267. doi: 10

Insert

ChR2-mCherry

Use

AAV

Expression

Mammalian

Promoter

Dlx

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_Xph15-eGFP-CCR5TC

Plasmid

#135528

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_Xph20-mEos3.2-CCR5TC

Plasmid

#135532

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mEos3.2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTU-DO-URA-C4

Plasmid

#160339

Purpose

High-copy expression vector for K. marxianus, uracil selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101102

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MMLV-RT for Split-PE (pEH45)

Plasmid

#190105

Purpose

Expresses MMLV-RT (full-length, from PE2) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT

Tags

bpNLS

Expression

Mammalian

Mutation

RT pentamutant from PE2 (D200N, T306K, W313F, T33…

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE81L-KOFP7

Plasmid

#160330

Purpose

This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

Depositor

Sang Taek Jung

Article

Ko et al J Agric Food Chem. 2020 May 27;68(21):58

Insert

KOFP7

Tags

6xHis tag

Expression

Bacterial

Promoter

T5

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAS-PE2-attB

Plasmid

#149550

Purpose

Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

UAS

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD_Psra-6::ChR2(H134R)::mCherry

Plasmid

#117419

Purpose

For selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

channel rhodopsin-2 with H134R mutation

Expression

Worm

Mutation

H134R

Promoter

sra-6

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge