We narrowed to 8,301 results for: gnal

1. dCas9-NS3-NLS/VPR

   Plasmid

   #112244

   Purpose

   Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

   Depositor

   John Ngo

   Article

   Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

   Insert

   dCas9-NS3-NLS/VPR
   

   Use

   Tags

   AU1 (N term on NS3) and HA (C term on NS3)

   Expression

   Mammalian

   Mutation

   NS3 mutant T54A

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF5B-FRT-AP-Smo-YFP-DEST

   Plasmid

   #49099

   Purpose

   Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Smo (Smo Mouse)
   

   Use

   Tags

   YFP and acceptor peptide (AP)

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPPI4-Strep-SNAP-PD-1

   Plasmid

   #223569

   Purpose

   Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ETV5_pet28a

   Plasmid

   #131639

   Purpose

   Bacterial expression of ETS gene ETV5

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV5 (ETV5 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

   Plasmid

   #173872

   Purpose

   Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5 DN-Fyn

   Plasmid

   #16033

   Depositor

   Filippo Giancotti

   Article

   Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

   Insert

   c-Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dominant negative: K299M

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pRK5-FLAG-FNIP1

   Plasmid

   #72292

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

   Insert

   FNIP1 (FNIP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCI-neo beta catenin S33Y

   Plasmid

   #16519

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   beta catenin (CTNNB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S33Y (SW48 mutation)

   Promoter

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7m-psCDK5

   Plasmid

   #89365

   Purpose

   A lenti-viral vector expressing photoswitchable CDK5 in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psCDK5 (CDK5 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL3.7m-psRaf1

    Plasmid

    #89364

    Purpose

    A lenti-viral vector expressing photoswitchable Raf1 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psRaf1 (RAF1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV5-rat ERK2-L73P/S151D

    Plasmid

    #40819

    Depositor

    Natalie Ahn

    Article

    Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

    Insert

    MAPK1 (Mapk1 Rat)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L73P, S151D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hGFAP-Pink Flamindo-WPRE-SV40

    Plasmid

    #178790

    Purpose

    AAV vector to express the red cAMP indicator in astrocytes

    Depositor

    Tetsuya Kitaguchi

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    Pink Flamindo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3puro-mGFP-FXR1a-sh5Resistant

    Plasmid

    #225448

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ELK1_pet28a

    Plasmid

    #131647

    Purpose

    Bacterial expression of ETS gene ELK1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CA-0059 CMVTO-SumIL2-rens-5GS-IL2Rb

    Plasmid

    #231224

    Purpose

    CageIL-2 (C-term 25 AA)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    SumIL2-rens-5GS-IL2Rb (IL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L80F, R81D, L85V, I86V, I92F

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-HA-FNIP1

    Plasmid

    #72293

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FNIP1 (FNIP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3.1-Flag-H2A K13-15R

    Plasmid

    #63563

    Purpose

    Expression of human H2A with K13R and K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 13 and Lysine 15 are now Arginine

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pEYFP-N1-A2BR

    Plasmid

    #37202

    Depositor

    Robert Tarran

    Article

    Watson et al FASEB J. 2011 Sep;25(9):2996-3003. Epub

    Insert

    A2BR (ADORA2B Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. V5-bArr2

    Plasmid

    #201493

    Purpose

    Expression of the beta-arrestin2 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    V5-bArr2 (ARRB2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRc/RSV Flag MKK3(glu)

    Plasmid

    #14670

    Depositor

    Roger Davis

    Article

    Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

    Insert

    MKK3(glu) (MAP2K3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-189Glu Thr-193Glu

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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