We narrowed to 4,893 results for: PIR

1. retro-gfp-puro vector

   Plasmid

   #58249

   Purpose

   Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86

   Plasmid

   #212538

   Purpose

   Encodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

   Plasmid

   #135812

   Purpose

   This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

   Depositor

   Nadia Mercader Huber

   Article

   Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

   Insert

   TagRFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA flag PGC1 (180-797)

   Plasmid

   #8943

   Depositor

   Bruce Spiegelman

   Article

   Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

   Insert

   PGC-1a (Ppargc1a Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197063

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-f:PGC1-3D

   Plasmid

   #47

   Depositor

   Bruce Spiegelman

   Article

   Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

   Insert

   PGC-1a (Ppargc1a Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Amino acids 262, 265, and 298 have been changed t…

   Promoter

   Available Since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pLEX307_PDHA2S293A

   Plasmid

   #115197

   Purpose

   Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PDHA2S293A (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   p.S293A

   Promoter

   EF1alpha

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-f:PGC1-L2/3A

   Plasmid

   #48

   Depositor

   Bruce Spiegelman

   Article

   Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

   Insert

   PGC-1a (Ppargc1a Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   LXXLL at aa140 is changed to AXXLL; LLXXL at aa 2…

   Promoter

   Available Since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

    Plasmid

    #128551

    Purpose

    Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-GSAS-deltaH69/V70

    Plasmid

    #171744

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid

    #117319

    Purpose

    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    hsa-miR-CTRL
    

    Use

    Transposon-mediated integration

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    miR-124 deleted

    Promoter

    Tet-inducible promoter

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gal4 PGC1 alpha L2/3A

    Plasmid

    #8898

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4 DBD

    Expression

    Mammalian

    Mutation

    LXXLL at aa140 is changed to AXXLL; LLXXL at aa 2…

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pTE3329

    Plasmid

    #107538

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K595R

    Promoter

    CMV and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pαH-S-GSAS-B.1.351.v5

    Plasmid

    #173790

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 5

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant version 5) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pαH-S-GSAS-D614G-N501Y

    Plasmid

    #171748

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-N501Y variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pαH-S-GSAS-D614G-E484K

    Plasmid

    #171751

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-E484K variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-E484K variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pαH-S-GSAS-D614G-K417N

    Plasmid

    #171752

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-K417N variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTE3330

    Plasmid

    #107534

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV and human U6

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pαH-S-GSAS-D614G-I692V

    Plasmid

    #171746

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-I692V variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-I692V variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge