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pmCherry-U6-RGS8

Plasmid

#140585

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RGS8

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-GTPBP2

Plasmid

#140586

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA GTPBP2

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVd2-Dr-TrkB

Plasmid

#127955

Purpose

Myr DrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-3xSAG-Dr-hel4-TrkB

Expression

Mammalian

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSKAP

Plasmid

#122702

Purpose

Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Type

Empty backbone

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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spoTD297A_NS1

Plasmid

#100915

Purpose

expresses spoTD297A (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

synpcc7942_1377 (spoT)

Use

Cyanobacteria cloning vector

Mutation

Changed D 297 to A

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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relA+_NS1

Plasmid

#100916

Purpose

expresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli

Use

Cyanobacteria cloning vector

Tags

FLAG

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Cas1

Plasmid

#122285

Purpose

Cas1 cloned in pHMGWA

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas1

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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BB3_arscen_URA3_Hygro

Plasmid

#118077

Purpose

low copy plasmid, neg. control

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJet1.2 HA6-Tag

Plasmid

#117126

Purpose

C-terminal HA-tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

6xHA Tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

6xHA tag

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Pk6-tag

Plasmid

#117127

Purpose

C-terminal Pk6-tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

6xPK tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

6xPK tag

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 'rncS

Plasmid

#117129

Purpose

smc gene upstream homology region

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

'rncS

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pll2.1

Plasmid

#107202

Purpose

PLL design 2.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

pll2.1 design

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pll2.2

Plasmid

#107203

Purpose

PLL design 2.2

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.2

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pll2.3

Plasmid

#107204

Purpose

PLL design 2.3

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.3

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pll2.4

Plasmid

#107205

Purpose

PLL design 2.4

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.4

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pll2.5

Plasmid

#107206

Purpose

PLL design 2.5

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.5

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pll3.1

Plasmid

#107207

Purpose

PLL design 3.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 3.1

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pll4.1

Plasmid

#107208

Purpose

PLL design 4.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 4.1

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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p249_LTJ_1kbFoxp3wtTemplate

Plasmid

#82664

Purpose

1kb HDR template for murine wildtype Foxp3

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine wildtype Foxp3

Use

Hdr template for gene editing

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p243_LTJ_4kbCD45.1Template

Plasmid

#82663

Purpose

4kb HDR template for murine CD45.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

4kb genomic DNA sequence from murine CD45.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC

Plasmid

#101828

Purpose

drives expression of kaiBC from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kanamycin casette and p0050 promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::glgP_gap1

Plasmid

#101845

Purpose

Replaces native promoter of glgP_gap1 with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

CmR casette followed by ptrc promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::zwf_fbp_opcA

Plasmid

#101846

Purpose

Replaces native promoter of zwf_fbp_opcA with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

sp/st resistance casette followed by ptrc promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::gnd

Plasmid

#101844

Purpose

drives expression of gnd using Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kan casette followed by ptrc promoter

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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zwf-fbp-opcA-

Plasmid

#101839

Purpose

deletes zwf_fbp_opcA locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_2333-2335 operon)

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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p250_LTJ_2kbFoxp3wtTemplate

Plasmid

#82665

Purpose

2kb HDR template for murine wildtype Foxp3

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine wildtype Foxp3

Use

Hdr template for gene editing

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS292

Plasmid

#73725

Purpose

SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-2

Plasmid

#72734

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-1

Plasmid

#72733

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-9

Plasmid

#72741

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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