We narrowed to 8,781 results for: construct
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Plasmid#71726Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RDDepositorInsertGal4 DBD HSF1 VP16 AD (HSF1 Human, Budding Yeast, Herpes Simplex Virus protein VP16)
ExpressionMammalianAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST15.1-hRPN13 (285-386)
Plasmid#61936PurposeExpression of hRPN13 (285-386) in E.coliDepositorInserthRPN13 (285-386) (ADRM1 Human)
TagsGSTExpressionBacterialMutationConstruct starts at residue 285, stop codon intro…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FUT8-1/1
Plasmid#91270PurposeProtein expression and purification of human SH3 domain construct FUT8-1/1DepositorInsertFUT8-1/1 (FUT8 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGEF6-1/1
Plasmid#91515PurposeProtein expression and purification of human SH3 domain construct ARHGEF6-1/1DepositorInsertARHGEF6-1/1 (ARHGEF6 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR
Plasmid#186745PurposedCas9-10XGcn4-P2A-mCherry expression constructDepositorInsertCas9-10XGcn4-P2A-mCherry
UseCRISPRExpressionMammalianAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry
Plasmid#179429PurposeDual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-5kb-mch
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNMT1 KO Neo
Plasmid#16649DepositorInsertDNMT1 knock-out construct (DNMT1 Human)
ExpressionBacterialAvailable SinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(306-593)-6xhis
Plasmid#192837PurposeExpress codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags6xhisExpressionBacterialPromoterT7Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ECON
Plasmid#82553PurposeEpigenetic effector construct for targeted methylationDepositorTagsHALO and mCherryExpressionMammalianMutationN-terminal photolyase homology region domain of C…PromoterCMVAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hNCL
Plasmid#103805PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOCK2-1/1
Plasmid#91482PurposeProtein expression and purification of human SH3 domain construct DOCK2-1/1DepositorInsertDOCK2-1/1 (DOCK2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AMPH-1/1
Plasmid#91556PurposeProtein expression and purification of human SH3 domain construct AMPH-1/1DepositorInsertAMPH-1/1 (AMPH Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DBNL-1/1
Plasmid#91409PurposeProtein expression and purification of human SH3 domain construct DBNL-1/1DepositorInsertDBNL-1/1 (DBNL Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1106
Plasmid#119249Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGEF16-1/1
Plasmid#91334PurposeProtein expression and purification of human SH3 domain construct ARHGEF16-1/1DepositorInsertARHGEF16-1/1 (ARHGEF16 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGEF26-1/1
Plasmid#91342PurposeProtein expression and purification of human SH3 domain construct ARHGEF26-1/1DepositorInsertARHGEF26-1/1 (ARHGEF26 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ITSN1-2/5
Plasmid#91385PurposeProtein expression and purification of human SH3 domain construct ITSN1-2/5DepositorInsertITSN1-2/5 (ITSN1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-ΔCT
Plasmid#64062Purposemyc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationDeletion of C-terminal Asp338–Ser443Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-pax3a-kcnj13-IRES-EGFP
Plasmid#164950PurposeTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoterpax3a promoter -5.4kAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-IRES-EGFP
Plasmid#164941PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR-tagRFP-T
Plasmid#103809Purpose'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_1_101
Plasmid#65728Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-101PromoterT7Available SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
Plasmid#61938PurposeCo-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coliDepositorTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 2-38 dele…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-2/2
Plasmid#91410PurposeProtein expression and purification of human SH3 domain construct GRB2-2/2DepositorInsertGRB2-2/2 (GRB2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
Plasmid#89890PurposeBAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.DepositorInsertHA-BirA-2A-citrine
UseUnspecifiedTagsCitrineAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hNCL
Plasmid#103806PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRKL-2/2
Plasmid#91364PurposeProtein expression and purification of human SH3 domain construct CRKL-2/2DepositorInsertCRKL-2/2 (CRKL Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 35s:PIF6:tNos (GB0170)
Plasmid#160606PurposeTU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switchDepositorInsertPIF6
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_129
Plasmid#65738Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-129PromoterT7Available SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ASAP1-1/1
Plasmid#91501PurposeProtein expression and purification of human SH3 domain construct ASAP1-1/1DepositorInsertASAP1-1/1 (ASAP1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOSTRIN-1/1-SV1/2
Plasmid#91323PurposeProtein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2DepositorInsertNOSTRIN-1/1-SV1/2 (NOSTRIN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3KBP1-1/3
Plasmid#91555PurposeProtein expression and purification of human SH3 domain construct SH3KBP1-1/3DepositorInsertSH3KBP1-1/3 (SH3KBP1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CD2AP-2/3
Plasmid#91391PurposeProtein expression and purification of human SH3 domain construct CD2AP-2/3DepositorInsertCD2AP-2/3 (CD2AP Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet02
Plasmid#78284PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRC2_p53DD
Plasmid#136520PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertp53DD (Trp53 Mouse)
UseEntry vector for gateway cloningTags3x FLAGMutationThis construct was created by PCR from the pBabe …Available SinceOct. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_100
Plasmid#65735Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-100PromoterT7Available SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNMBP-6/6
Plasmid#91489PurposeProtein expression and purification of human SH3 domain construct DNMBP-6/6DepositorInsertDNMBP-6/6 (DNMBP Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_KALRN-1/4
Plasmid#91283PurposeProtein expression and purification of human SH3 domain construct KALRN-1/4DepositorInsertKALRN-1/4 (KALRN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-Med4 pBacPAK8
Plasmid#15364DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CD2AP-1/3
Plasmid#91398PurposeProtein expression and purification of human SH3 domain construct CD2AP-1/3DepositorInsertCD2AP-1/3 (CD2AP Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only