We narrowed to 13,043 results for: SHI
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Plasmid#235740PurposeProtein expression of human RAB5A Q79,C19S,C63S,S84TAG, 1-212-His6 in bacteria for unnatural amino acid incorporationDepositorInsertRAB5A (RAB5A Human)
UseTags6xHisExpressionBacterialMutationQ79, C19S, C63S, S84TAG, aa 1-212PromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJB78
Plasmid#235738PurposeProtein expression of GST-TEV-HsRAB1A Q70L C26S C126S in bacteria for maleimide conjugationDepositorInsertRAB1A (RAB1A Human)
UseTagsGST-TEVExpressionBacterialMutationQ70L C26S C126S, aa 1-204PromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ HA BEX3
Plasmid#231004PurposeTransient overexpression of human BEX3 in mammalian cellsDepositorInsertBEX3 (BEX3 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2 3xFLAG FEM1A
Plasmid#231001PurposeTransient overexpression of mouse FEM1A in mammalian cellsDepositorInsertFEM1A (Fem1a Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-MUT-P2A-GFP
Plasmid#229406PurposeLentiviral or overpexression of cDNADepositorInsertMKI-MUT-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationinteraction mutant L109G; L143G (Control mutant)PromoterEFSAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VPS13B^Halo
Plasmid#224584Purposecodon optimized Halo tagged VPS13B for expression in mammalian cellsDepositorInsertVPS13B^Halo (VPS13B Human)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-MUT-P2A-GFP-DOX-N
Plasmid#229408PurposeLentiviral or overpexression of cDNADepositorInsertMKI-MUT-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationinteraction mutant L109G; L143G (Control mutant)PromoterTRE3GAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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ABEmax-Puro V2
Plasmid#226955PurposeABEmax plasmid enabling A:T to G:C base editing using a single plasmid.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-CtoS-FLAG
Plasmid#220876PurposeExpressing METTL17-FLAG_Cys to Ser mutantsDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged Cys to SerPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-LYRMut-FLAG
Plasmid#220877PurposeExpressing METTL17-LYRMut-FLAGDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged the LYP domain to AAAPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-455-WT
Plasmid#215881PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-376a1(3p)-I-type
Plasmid#215883PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-589(5p)-I-type
Plasmid#215884PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-589(3p)-I-type
Plasmid#215885PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-mmPCSK9
Plasmid#205634PurposereRNA guide targeting Mus Musculus PCSK9DepositorInsertreRNA-mmPCSK9 (Pcsk9 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-L
Plasmid#223967PurposeAnti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd
Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53 P428L
Plasmid#221321PurposeDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transductionDepositorInsertIRSp53 (Baiap2 Mouse)
UseRetroviralTags3xFLAGExpressionMutationP428LPromoterTRE3GAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53 S269D
Plasmid#221319PurposeDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transductionDepositorInsertIRSp53 (Baiap2 Mouse)
UseRetroviralTags3xFLAGExpressionMutationS269DPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-GMIP
Plasmid#221318PurposeDox-inducible expression of GMIP in mammalian cells by retroviral transductionDepositorInsertGMIP (Gmip Mouse)
UseRetroviralTags3xFLAGExpressionMutationPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53
Plasmid#221317PurposeDox-inducible expression of IRSp53 in mammalian cells by retroviral transductionDepositorInsertIRSp53 (Baiap2 Mouse)
UseRetroviralTags3xFLAGExpressionMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-ARHGAP45
Plasmid#221301PurposeExpression of ARHGAP45 (pLxIS) in mammalian cells by retroviral transductionDepositorInsertARHGAP45 (pLxIS) (Hmha1 Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-IRSp53(pLxIS)
Plasmid#221336PurposeOverexpression of IRSp53 pLxIS domain in bacteriaDepositorInsertIRSp53 pLxIS domain (Baiap2 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-DZIP1L
Plasmid#221290PurposeExpression of DZIP1L (pLxIS) in mammalian cells by retroviral transductionDepositorInsertDZIP1L (pLxIS) (Dzip1l Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TRIF(pLxIS) S209A
Plasmid#221245PurposeExpression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transductionDepositorInsertTRIF pLxIS domain (1-222) (Ticam1 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationS209APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TRIF(pLxIS) L193A
Plasmid#221246PurposeExpression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transductionDepositorInsertTRIF pLxIS domain (1-222) (Ticam1 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationL193APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5Q-GS3
Plasmid#204746PurposeExpression of Cas9 and human H2AX with the K5Q mutationDepositorInsertH2AX K5Q (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K5Q mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5R-GS3
Plasmid#204747PurposeExpression of Cas9 and human H2AX with the K5R mutationDepositorInsertH2AX K5R (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K5R mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134L-GS3
Plasmid#204750PurposeExpression of Cas9 and human H2AX with the K134L mutationDepositorInsertH2AX K134L (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K134L mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134M-GS3
Plasmid#204751PurposeExpression of Cas9 and human H2AX with the K134M mutationDepositorInsertH2AX K134M (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K134M mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134A-GS3
Plasmid#204752PurposeExpression of Cas9 and human H2AX with the K134A mutationDepositorInsertH2AX K134A (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K134A mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT46
Plasmid#218724PurposeExpresses N-terminally EGFP-tagged IFT46 in mammalian cellsDepositorInsertIFT46 (IFT46 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT25
Plasmid#218721PurposeExpresses N-terminally EGFP-tagged IFT25 in mammalian cellsDepositorInsertIFT25 (HSPB11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC
Plasmid#213535PurposeExpresses the split transcription factors in cultured neuron and mouse brainDepositorInsertSspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA
UseAAVTagsEGFP and HAExpressionMammalianMutationPromoterSynapsinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(HA)
Plasmid#195023PurposeHA-tagged Creb5 in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5Â (full length, 508aa) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128)
Plasmid#195031PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PFKM_sgRNA1
Plasmid#201610PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPFKM (PFKM Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PFKM_sgRNA2
Plasmid#201611PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPFKM (PFKM Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-SNRNP70_sgRNA1
Plasmid#201620PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertSNRNP70 (SNRNP70 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-SNRNP70_sgRNA2
Plasmid#201621PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertSNRNP70 (SNRNP70 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-NDUFB5_sgRNA1
Plasmid#201606PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertNDUFB5 (NDUFB5 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-NDUFB5_sgRNA2
Plasmid#201607PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertNDUFB5 (NDUFB5 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PABPC1_sgRNA2
Plasmid#201609PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPABPC1 (PABPC1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgVRK1-hygro
Plasmid#199640PurposesgRNA guide against VRK1DepositorInsertN/A (VRK1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-In18runon-PTPN22
Plasmid#195377PurposeA human PTPN22 mutant (intron 18-runon) in Gateway pDONR221DepositorInsertPTPN22 (PTPN22 Human)
UseGateway donor vectorTagsExpressionMutationIntron 18-runon PTPN22PromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-In18runon-PTPN22
Plasmid#195379PurposeMammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationIntron 18-runon PTPN22PromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only