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pcDNA3.1_ADAR2DD_E488Q

Plasmid

#205598

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

Depositor

Marc Friedlander

Publication

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ADAR2 deaminase domain (ADARB1 Human)

Expression

Mammalian

Mutation

E488Q hyperactive mutation

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2

Plasmid

#85658

Purpose

retroviral expression of human SMYD2

Depositor

Julien Sage

Publication

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

LTR

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-EXOSC10

Plasmid

#23674

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EXOSC10 (EXOSC10 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Publication

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28sumo-hRig-I-Full-length

Plasmid

#210827

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Publication

Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

Insert

hRIG-I (DDX58) Full length protein (RIGI Human)

Expression

Bacterial

Mutation

NA

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Publication

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgKEAP1-2

Plasmid

#186459

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PGKp-GFP-TAZ

Plasmid

#174176

Purpose

Lentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.

Depositor

Rod Bremner

Publication

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

PGK

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Publication

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-BirA-FLAG

Plasmid

#105786

Purpose

Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

Depositor

Andrzej Dziembowski

Publication

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-BirA-FLAG

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-LacZ

Plasmid

#18816

Depositor

Connie Cepko

Publication

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SV40 basal promoter-LacZ

Expression

Mammalian

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pT-FLAG

Plasmid

#31385

Depositor

Yegor Vassetzky

Publication

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Type

Empty backbone

Tags

FLAG

Expression

Mammalian

Available since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pME-QF2

Plasmid

#83307

Purpose

codes for QF2 (truncated QF) in a gateway middle entry backbone.

Depositor

Marnie Halpern

Insert

QF

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_hUmiRc2

Plasmid

#25747

Purpose

Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[CoChR-GFP]

Plasmid

#62724

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

Plasmid

#55221

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

StrepII and msfGFP

Expression

Insect

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

Plasmid

#48297

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

StrepII and msfGFP

Expression

Insect

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pSL690

Plasmid

#47753

Purpose

Expresses dCas9-VP64 fusion

Depositor

Keith Joung

Publication

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HIR Y1158,1162,1163F

Plasmid

#24054

Depositor

Frederick Stanley

Publication

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1158F, Y1162F, Y1163F

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only