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Plasmid#35519DepositorInsertChR1-ChR2 Chimera
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
dimericTomato-1xwGBD
Plasmid#176099PurposeVisualization of active Cdc42DepositorInsertWAS (WAS Human)
UseLentiviralTagsExpressionMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG FUCCI
Plasmid#175266PurposeBicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesisDepositorInsertFUCCI
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin-pcDNA3
Plasmid#45769DepositorInserthE-cadherin (CDH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo-CnB
Plasmid#191965PurposeExpression of CnB (calcineurin subunit beta).DepositorInsertCalcineurin subunit beta (PPP3CB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
EcSTH-LbNOX
Plasmid#211924PurposeThis plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.DepositorInsertEcSTH-LbNOX
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
Plasmid#35152DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5, pTrcAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET YBX-1
Plasmid#13035DepositorInsertYBX1 (YBX1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTP341 pHAGE2 CMV TagBFP-3xFLAG
Plasmid#199391PurposeLentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cellsDepositorInsertTagBFP-3xFLAG
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shSHMT2
Plasmid#106319PurposeExpress shRNA targeting SHMT2DepositorInsertshRNA targeting SHMT2
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL3-FLAG
Plasmid#15820DepositorInsertCullin3 (CUL3 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N418PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(-30)GFP
Plasmid#62936PurposeExpression of 6xHis-(-30)GFP in bacterial cellsDepositorInsert(-30)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-PHYTipi
Plasmid#53283PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT525
Plasmid#131392PurposeL1RP retrotransposition reporterDepositorInsertL1RP GFP-AI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-SCP2
Plasmid#31336DepositorInsertsterol carrier protein 2 (SCP2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGR
Plasmid#46002PurposeReporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinoseDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
miniCMV eGFP hPGK BSD
Plasmid#188519PurposeBackbone for CRISPR activation (CRISPRa) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Nck1
Plasmid#45903DepositorInsertNck-1 (NCK1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR2P_P474S_P493N
Plasmid#198695PurposeExpression of human FXR2 protein in E. coliDepositorInsertFXR2P (FXR2 Human)
UseTagsMBPExpressionBacterialMutationChanged proline 474 to serine and proline 493 asp…PromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTetR-LasR-pLuxR-GFP
Plasmid#171159PurposeSensing Device with Reporter proteinDepositorInsertslasR
gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLux and pTetAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6610 MSCV-IP N-HAonly TIMP1
Plasmid#35009DepositorInsertTIMP1 (TIMP1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT3-Neo-EF1a-GFP
Plasmid#69134PurposeSleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promotersDepositorTypeEmpty backboneUseSleeping beauty transposonTagsExpressionMammalianMutationPromoterU3MSCVAvailable sinceJan. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
groSL-GFP
Plasmid#109389PurposeGFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpgroSL-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R-6His
Plasmid#58979Purposeexpresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsHisExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9
Plasmid#188489PurposeExpresses FLAG tagged SpCas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA14 (MSW#503)
Plasmid#208069PurposeExpression of GFP-tagged hsRPA3 in human cellsDepositorInsertRPA3 (RPA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Blasto_zhang2.0
Plasmid#167912PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCk2
Plasmid#136696PurposeAcceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)
Plasmid#191451PurposeVisualizing endogenous & active Cdc42DepositorInsertN-WASP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup93
Plasmid#87336PurposeTo express human Nup93 in mammalian cellsDepositorInsertNUP93 (NUP93 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA5-Halo
Plasmid#175326PurposeProduction of HaloTag fusion proteinDepositorInsertHSPA5 (HSPA5 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pADV-Sec61b-TurboID
Plasmid#179143PurposeRecombinant adenoviral plasmid for Sec61b-TurboID expressionDepositorInsertSec61b-V5-TurboID
UseAdenoviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEHlyA5
Plasmid#168298PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyADepositorInsertN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
UseTagsE-tag, HA-tag, His-tag, Prescission site, and VHH…ExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-CCre
Plasmid#106369PurposeC-terminal part of the split-Cre SystemDepositorInsertCCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only