Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,121 results for: Sis

Showing: 9751 - 9800 of 23121 results

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pSF-NgPglO

Plasmid

#198204

Purpose

pSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

NgPglO

Tags

Flag tag

Expression

Bacterial

Promoter

pBAD

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-AcrA

Plasmid

#198214

Purpose

pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

AcrA

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pOBD2

Plasmid

#196826

Purpose

Yeast two-hybrid ; Gal4 DNA binding domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Tags

Gal4 DNA binding domain

Expression

Yeast

Promoter

ADH

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTet-Bxb1-CFP

Plasmid

#195374

Purpose

Expresses Bxb1 integrase fused with CFP for fluorescence

Depositor

Richard Murray

Article

Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

Insert

Bxb1 Integrase Fused With deCFP

Use

Synthetic Biology

Tags

deCFP

Promoter

pTet

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (E78K)

Plasmid

#197002

Purpose

Mammalian inducible expression construct of myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

E78K

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (W22R)

Plasmid

#197004

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

W22R

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (E47K)

Plasmid

#197003

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

E47K

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W131G SPOP 1-374

Plasmid

#197019

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W131G

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E78K SPOP 1-374

Plasmid

#197018

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E78K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E47K SPOP 1-374

Plasmid

#197017

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E47K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W22R SPOP 1-374

Plasmid

#197016

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W22R

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37U

Plasmid

#196659

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37U)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37U expression cassette

Expression

Bacterial

Mutation

A37U in tRNAfMet

Promoter

metY native promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37G

Plasmid

#196658

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37G)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37G expression cassette

Expression

Bacterial

Mutation

A37G in tRNAfMet

Promoter

metY native promoter

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN3-L676EG683E-del460-465_U

Plasmid

#147673

Purpose

Insect Expression of DmPAN3-L676EG683E-del460-465

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3-L676EG683E-del460-465 (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN3-L676EG683E-del460-465_U

Plasmid

#147658

Purpose

Insect Expression of DmPAN3-L676EG683E-del460-465

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3-L676EG683E-del460-465 (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-C421F_T

Plasmid

#147561

Purpose

Bacterial Expression of DmPAN3_349-790-C421F

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-C421F (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-C421FI555F_T

Plasmid

#147562

Purpose

Bacterial Expression of DmPAN3_349-790-C421FI555F

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-C421FI555F (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-F658EL662EL665E_T

Plasmid

#147563

Purpose

Bacterial Expression of DmPAN3_349-790-F658EL662EL665E

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-F658EL662EL665E (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-I555F_T

Plasmid

#147564

Purpose

Bacterial Expression of DmPAN3_349-790-I555F

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-I555F (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-L676EG683E_T

Plasmid

#147565

Purpose

Bacterial Expression of DmPAN3_349-790-L676EG683E

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-L676EG683E (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-M651F_T

Plasmid

#147566

Purpose

Bacterial Expression of DmPAN3_349-790-M651F

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-M651F (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-M651FP652G_T

Plasmid

#147567

Purpose

Bacterial Expression of DmPAN3_349-790-M651FP652G

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-M651FP652G (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-M651H_T

Plasmid

#147568

Purpose

Bacterial Expression of DmPAN3_349-790-M651H

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-M651H (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmPAN3_349-790-M651HP652G_T

Plasmid

#147569

Purpose

Bacterial Expression of DmPAN3_349-790-M651HP652G

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-M651HP652G (CG11486 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-RLuc-HsAsb9-3UTR_X

Plasmid

#147949

Purpose

Mammalian Expression of HsAsb9-3UTR

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsAsb9-3UTR (ASB9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-I26 pUBQ10 GG0-GG1

Plasmid

#195902

Purpose

Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10 (UBQ10 Mustard Weed)

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p8271 LentiCRISPR v2 hygro sgSIGIRR-4

Plasmid

#193980

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR3_BC2

Plasmid

#191857

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgPIGA guide 1

Plasmid

#193610

Purpose

PIGA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGA guide 1 (PIGA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgPIGA guide 2

Plasmid

#193611

Purpose

PIGA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGA guide 2 (PIGA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgPIGW guide 1

Plasmid

#193612

Purpose

PIGW knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGW guide 1 (PIGW Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgPIGW guide 2

Plasmid

#193613

Purpose

PIGW knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPIGW guide 2 (PIGW Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX317 TFAP4

Plasmid

#193616

Purpose

Expresses TFAP4

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

TFAP4 (TFAP4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgNFkBIA guide 1

Plasmid

#193595

Purpose

NFkBIA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkBIA guide 1 (NFKBIA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgNFkBIA guide 2

Plasmid

#193596

Purpose

NFkBIA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkBIA guide 2 (NFKBIA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgSNRPA guide 1

Plasmid

#193598

Purpose

SNRPA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSNRPA guide 1 (SNRPA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgSNRPA guide 2

Plasmid

#193599

Purpose

SNRPA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSNRPA guide 2 (SNRPA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgPGD guide 1

Plasmid

#193602

Purpose

PGD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPGD guide 1 (PGD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003 sgPGD guide 2

Plasmid

#193603

Purpose

PGD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgPGD guide 2 (PGD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003_sgTFAP4 guide 1

Plasmid

#193586

Purpose

TFAP4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgTFAP4 guide 1 (TFAP4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003_sgTFAP4 guide 2

Plasmid

#193587

Purpose

TFAP4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgTFAP4 guide 2 (TFAP4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR3_BC6

Plasmid

#191861

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR3_BC5

Plasmid

#191860

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR3_BC4

Plasmid

#191859

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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