We narrowed to 4,010 results for: SPL

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pDragon-p21

Plasmid

#155016

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Cdkn1a (Cdkn1a Mouse)

Use

Mouse Targeting

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSD025A

Plasmid

#218128

Purpose

Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of gIII-neg

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
M13 pIII-neg

Tags

scP22

Mutation

W384A, Y386A

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast

Plasmid

#171951

Purpose

All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-myc-RRBP1-WPRE-UbC-Emerald

Plasmid

#225942

Purpose

Lentiviral vector plasmid expressing human ribosome binding protein 1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis, Evan Reid

Inserts

RRBP1 (RRBP1 Human)
Emerald

Use

Lentiviral

Tags

myc-tag

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v1 IQSEC1 WT

Plasmid

#162016

Purpose

Expression of IQSEC1 splice variant 1

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v2 IQSEC1 WT

Plasmid

#162017

Purpose

Expression of IQSEC1 splice variant 2

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDN-D2irC6kwh

Plasmid

#44725

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
mCherry

Use

Synthetic Biology; Expression regulator/reporter

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

Plasmid

#69049

Purpose

lentiviral expression of IKZF2 variant 2 with FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2 (IKZF2 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

splice variant 2

Promoter

CAG

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

Plasmid

#69051

Purpose

lentiviral expression of IKZF1 with HA tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

splice variant 2

Promoter

UBC

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-NEDD1-gTBD

Plasmid

#196858

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-EGFP-NEDD1-gTBD

Plasmid

#196857

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EGFP-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v3 IQSEC1 WT

Plasmid

#162018

Purpose

Expression of IQSEC1 splice variant 3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v4 IQSEC1 WT

Plasmid

#162019

Purpose

Expression of IQSEC1 splice variant 4

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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3322_pETcon_SARS2_Omicron-BA2

Plasmid

#184409

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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P2_HNF4A8

Plasmid

#31094

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 8 derived from promoter P2

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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PLUNC-bio-His

Plasmid

#53428

Purpose

Expresses full-length Palate lung and nasal epithelium clone protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PLUNC (BPIFA1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CDreV

Plasmid

#140133

Purpose

can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2-C106R

Plasmid

#31123

Depositor

Gerhart Ryffel

Article

Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

Insert

HNF4A (HNF4A Human)

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2, mutation C106R. Wild type seq…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CXCR4-VN

Plasmid

#98964

Purpose

FLAG-tagged human CXCR4 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

FLAG (dykdddd) epitope tag, Signal/leader sequenc…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-VC

Plasmid

#98967

Purpose

myc-tagged human CXCR4 fused to C-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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