We narrowed to 49,036 results for: ang

1. CMV-sgE1TSS-short-3'box

   Plasmid

   #92165

   Purpose

   sgRNA expression vector for Evx1as RNA tethering assay

   Depositor

   Xiaohua Shen

   Article

   Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

   Insert

   Evx1as short isoform
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-GST-DAXX-His

   Plasmid

   #175781

   Purpose

   DAXX protein for baculovirus expression system

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT077

   Plasmid

   #180511

   Purpose

   Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   PlmCasX
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-HA PSPC1

   Plasmid

   #101764

   Purpose

   Expresses PSPC1 in mammalian cells

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   paraspeckle component 1 (PSPC1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. eTd-CBE

   Plasmid

   #196601

   Purpose

   Mammalian expression of eTd-CBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Use

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-EZH2

   Plasmid

   #28060

   Depositor

   Mien-Chie Hung

   Article

   Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ACBE

   Plasmid

   #204607

   Purpose

   Mammalian expression of adenine-to-cytosine base editor ACBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E-3x125bp-mnx1

   Plasmid

   #169043

   Purpose

   5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   Mnx1 enhancer (3x) (mnx1 Zebrafish)
   

   Use

   Gateway entry cloning vector

   Tags

   Expression

   Mutation

   Promoter

   3xMnx1

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pinducer20-BSD-scFv-sfGFP-DNMT3A1

    Plasmid

    #115780

    Purpose

    targeted DNA methylation tool

    Depositor

    Margaret Goodell

    Article

    Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

    Insert

    scFv-sfGFP-DNMT3A1 (DNMT3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-G88P7-rM3Ds-2A-EYFP

    Plasmid

    #213970

    Purpose

    AAV vector with G88P7 promoter in order to induce robust expression of rM3Ds in MSNs

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Insert

    rM3Ds
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    G88P7

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO (pEHA1339)

    Plasmid

    #209088

    Purpose

    Backbone plasmid for Tet Responsive transgene expression, FRT integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. eTd-CBEm

    Plasmid

    #196602

    Purpose

    Mammalian expression of eTd-CBEm

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eTd-CBEa

    Plasmid

    #196603

    Purpose

    Mammalian expression of eTd-CBEa

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHSN401

    Plasmid

    #50588

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    maize codon optimized

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgRNA-Cre-GpNLuc

    Plasmid

    #208382

    Purpose

    Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBUN411

    Plasmid

    #50581

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    maize codon optimized

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-MTS-G3635A-L17-FZY2-S100N-T26I&T77I-UGI-Puro

    Plasmid

    #209645

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100N-T26I&T77I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-TRE-DIO-GCaMP6s

    Plasmid

    #183809

    Purpose

    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene TetO promoter-DIO/flex-GCaMP6
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2-sgDHODH-4

    Plasmid

    #186022

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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