We narrowed to 16,181 results for: nes
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Plasmid#21479PurposeExpression of KifC2-GFP in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKinesin family member C2 (Kifc2 Mouse)
TagsGFPExpressionMammalianAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
N370S GBA pcDNA3.1
Plasmid#188583PurposeEctopic expression N370S GBADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGBA (GBA1 Human)
ExpressionMammalianMutationN370SPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2 RANK-L
Plasmid#108571PurposeEncodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tagDepositorInsertRANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
TagsGSTExpressionMammalianMutationaa158-aa316PromoterPtac (trp/lac)Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMD19T-psba1-TetR-PL22-dCas9-SpR
Plasmid#73223PurposeContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorPublicationInsertdCas9 from S. pyogenes
Tagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPL22Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
P336-FBP_11
Plasmid#139702PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing LuzDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
UseLuciferaseExpressionPlantPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-tdTomato_L3-L2
Plasmid#113708PurposeSingle pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.DepositorInsertCBF (notch dependent promoter)
UseGateway CloningExpressionMammalianAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CDC34
Plasmid#25598PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pX330-Flag-deSpCas9
Plasmid#92114PurposeExpression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdead/inactive eSpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840A, K848A, K1003A, R1060APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 ZoAl-ENT N-1F (R1103A)
Plasmid#216548PurposeFor expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
TagsFlagExpressionMammalianAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-TAGLN2
Plasmid#31569DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJA258
Plasmid#21511DepositorInsert(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
UseGateway CloningAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A+B
Plasmid#149370PurposeExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA-A
sgRNA-B
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p817-mgammaFcry-EGFP
Plasmid#23156DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmouse gammaF crystallin promoter:EGFP (eGFP mouse gammaF promoter:EGFP, Mouse)
UseMulticloning site flanked by i-scei sitesMutationmouse gammaF crystallin promoter (-225 - +73) pre…Available sinceApril 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-hNG2-GFP
Plasmid#183910PurposeExpression of GFP under the human NG2 promoterDepositorAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IDG_TMEM63B_OE_1
Plasmid#161682PurposeOverexpresses TMEM63BDepositorAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
BC1177-pHR-SFFV-GFP(1-10)-NLS
Plasmid#164039PurposeExpression of GFP1-10 in mammalian cellsDepositorInsertGFP(1-10)
UseLentiviralExpressionMammalianPromoterSFFVAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-3xFLAG-hSpCas9
Plasmid#162161PurposeExpresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTags3xFLAGExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by