We narrowed to 8,316 results for: Set

1. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR_full-site

   Plasmid

   #186277

   Purpose

   Reporter plasmid for full-site selection assay

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAP72_MS2

   Plasmid

   #220623

   Purpose

   Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Type

   Empty backbone

   Use

   Shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGD236

   Plasmid

   #219497

   Purpose

   pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   sfGFP, Eco1 msr, Eco1 RT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGD248

   Plasmid

   #219498

   Purpose

   Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAD6

   Plasmid

   #134349

   Purpose

   E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

   Depositor

   Seth Darst

   Article

   Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

   Insert

   mfd (mfd E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-LRF

   Plasmid

   #66936

   Purpose

   Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

   Depositor

   Alvaro Monteiro

   Article

   Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV-40

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHMattR3-attR4-GFP

   Plasmid

   #196247

   Purpose

   pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSkipFlox

   Plasmid

   #85797

   Purpose

   P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   split Cre recombinase
   

   Use

   Cre/Lox; P. falciparum expression

   Tags

   FKBP, FRB*

   Expression

   Mutation

   Promoter

   Pfcrt

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgDonor_lentiGuide

    Plasmid

    #184168

    Purpose

    LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Insert

    sgDonor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ChrII_ttTi5605_loxP_myo2_neoR

    Plasmid

    #194056

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ttTi5605
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAbaMCi-GFP-gfp_pJMP2768

    Plasmid

    #208889

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-eSIBR

    Plasmid

    #71665

    Purpose

    intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)

    Depositor

    Philip Washbourne

    Article

    Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

    Insert

    intronic eSIBR cassette
    

    Use

    RNAi; Multi-site gateway

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMyc-CMV1

    Plasmid

    #53582

    Purpose

    mammalian expression vector with Myc tag

    Depositor

    Linda Yu

    Article

    Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAH25-SceI

    Plasmid

    #129389

    Purpose

    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Chloramphenicol acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    CAT promoter

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCL.140

    Plasmid

    #184995

    Purpose

    Express Cas9 in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TetOn-3g

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3_alpha1-AtUBQ10:GFP-T35S

    Plasmid

    #211836

    Purpose

    Plant transient expression cassette for GFP (control) under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    GFP control
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CasRX_Pac

    Plasmid

    #194803

    Purpose

    CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

    Depositor

    Alexander Paredez

    Article

    Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

    Insert

    CasRX
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)

    Plasmid

    #167357

    Purpose

    Integrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    TDH3p:his6-mKate2-LipPks1-IDP1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-eSIBR

    Plasmid

    #71664

    Purpose

    EGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backbone

    Depositor

    Philip Washbourne

    Article

    Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

    Insert

    EGFP coding sequence followed by eSIBR cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pATF4-donor

    Plasmid

    #113805

    Purpose

    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP

    Depositor

    Kevin White

    Insert

    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs140428747

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LT0

    Plasmid

    #210551

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT0::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LT1

    Plasmid

    #210552

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT1::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LT2

    Plasmid

    #210553

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT2::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDonorTp

    Plasmid

    #211788

    Purpose

    pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR

    Depositor

    Silvia Cardona

    Article

    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

    Insert

    dhfr
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)

    Plasmid

    #167356

    Purpose

    Integrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    TDH3p:his6-mKate2-MBP-IDP1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BASIC_SEVA_66.11

    Plasmid

    #194213

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pSTK120-mCMV-TK

    Plasmid

    #22054

    Depositor

    Maria Castro

    Article

    King et al J Virol. 2008 May . 82(9):4680-4.

    Insert

    mCMV-TK-pA expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pOCC82

    Plasmid

    #118862

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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