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We narrowed to 17,856 results for: Mut

Showing: 7841 - 7880 of 17856 results

  1. pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

    Plasmid
    #229691
    Purpose
    Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)
    Depositor
    Alpha Yap
    Article
    Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.
    Inserts
    shRNA for hCavin1 (CAVIN1 Human)
    mutant cavin1b (Del4UC1) (cavin1b Zebrafish)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Del4UC1
    Promoter
    LTR viral promoter
    Available Since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    229691-plasmid-map-sequence-id-459544

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  3. pWZL-Blast FAP S624A

    Plasmid
    #207402
    Purpose
    Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.
    Depositor
    Jing Yang
    Article
    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi
    Insert
    Fibroblast Activation Protein Alpha (FAP Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    changed Serine 624 to Alanine. It's a protea…
    Promoter
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207402-plasmid-map-sequence-id-414821

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  4. pWZL-Blast FAP A657S

    Plasmid
    #207403
    Purpose
    Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.
    Depositor
    Jing Yang
    Article
    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi
    Insert
    Fibroblast Activation Protein Alpha (FAP Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    changed Alanine 657 to Serine. It's an endop…
    Promoter
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207403-plasmid-map-sequence-id-414820

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  9. pNK845

    Plasmid
    #219746
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Promoter
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219746-plasmid-map-sequence-id-437111

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  10. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

    Plasmid
    #218426
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.
    Insert
    GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Human, Entacmaea quadricolor)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of both…
    Promoter
    CMV
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218426-plasmid-map-sequence-id-434963

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  11. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)

    Plasmid
    #218425
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.
    Insert
    GABARAP(G116A)-mTagBFP2-GABARAP(5X) (GABARAP Human, Entacmaea quadricolor)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of down…
    Promoter
    CMV
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218425-plasmid-map-sequence-id-434958

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  12. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP

    Plasmid
    #212107
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.
    Insert
    GABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of upst…
    Promoter
    CMV
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    212107-plasmid-map-sequence-id-420139

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  25. pDEST-Myc-FBW7]K404R

    Plasmid
    #200715
    Purpose
    The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW7 K404 is mutated to Alanine.
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200715-plasmid-map-sequence-id-394919

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  26. pDEST-Myc-FBW7K404/412/R

    Plasmid
    #200716
    Purpose
    The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW7 K404 and K412 mutated to Alanines.
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200716-plasmid-map-sequence-id-394895

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  28. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

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  34. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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  38. LeGO-iBSD-5LO-E376Q

    Plasmid
    #182742
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase E376Q (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    enzymatically inactive 5-LO E376Q mutant, codon o…
    Promoter
    SFFV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182742-plasmid-map-sequence-id-367803

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  40. pBoBi-hPEN2 2A

    Plasmid
    #183640
    Purpose
    Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).
    Depositor
    Sheng-cai Lin
    Article
    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:
    Insert
    PEN2 (PSENEN Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    residues 35 and 40 of PEN2 mutated to alanine
    Promoter
    CMV
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183640-plasmid-map-sequence-id-363111

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Showing: 7841 - 7880 of 17856 results