We narrowed to 26,374 results for: sta

1. AA0250-mCherry-2a-hM4D-nrxn1a rev

   Plasmid

   #60544

   Purpose

   Co-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus

   Depositor

   Scott Sternson

   Article

   Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

   Inserts

   mCherry
   muscarinic receptor 4, axon targeted variant (CHRM4 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   2xHA

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTarget

   Plasmid

   #214052

   Purpose

   Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Target sequence
   

   Use

   Target rna plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHochTypeIII

   Plasmid

   #214039

   Purpose

   Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Cas10
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTTCC

   Plasmid

   #164264

   Purpose

   Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

   Depositor

   Gudmundur Hreggvidsson

   Article

   Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

   Inserts

   Cas9
   gRNA scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pcel and trc

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-IRES-BSD

   Plasmid

   #209751

   Purpose

   Lentiviral transfer plasmid to express the blasticidin resistance gene

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ALK3 wt

   Plasmid

   #80873

   Purpose

   mammalian expression of ALK3 WT

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK3 (BMPR1A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

   Plasmid

   #155307

   Purpose

   Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT Smarca4-V5 IRES-puro

   Plasmid

   #107058

   Purpose

   Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Jerry Crabtree, Courtney Hodges

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLS13-IL-10

    Plasmid

    #209128

    Purpose

    Transient mammalian expression of IL-10

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    IL-10 (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX NPC1-FLAG-TiD

    Plasmid

    #199748

    Purpose

    Lentiviral construct to stably express NPC1 TurboID

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Flag and TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG R2pH-LAMP1-3xFLAG

    Plasmid

    #157940

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Chick beta actin pomoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ALK2 Q207D

    Plasmid

    #80871

    Purpose

    mammalian expression of ALK2 Q207D

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK2 (ACVR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q207D

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pDONOR223 RpH-LAMP1-3xFLAG

    Plasmid

    #163019

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-FLAG-NAT10-siR-G641E

    Plasmid

    #59366

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    NAT10 NM_024662 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    G641E and silent mutations to render the cDNA res…

    Promoter

    CMV-tet

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)/GUG-DAP5-3XFLAG

    Plasmid

    #127330

    Purpose

    Express 3xFLAG tagged DAP5 protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    DAP5 (EIF4G2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiV_Neo_POU2F3_CR

    Plasmid

    #122175

    Purpose

    Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations for gRNA resistance (882g-c, 8…

    Promoter

    EFS

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. hCXCR4-LSSmOrange

    Plasmid

    #110197

    Purpose

    Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

    Insert

    hCXCR4 (CXCR4 Human)
    

    Use

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. retro-puro vector

    Plasmid

    #58250

    Purpose

    Retroviral expression vector encoding an empty IRES-Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FRT_htr2c(c)-GFP

    Plasmid

    #79677

    Purpose

    serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    serotonin receptor 2C (HTR2C Human)
    

    Use

    Tags

    EGFP in Frame

    Expression

    Mammalian

    Mutation

    splice site of exon Vb in consensus

    Promoter

    CMV

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only