We narrowed to 27,330 results for: sta

1. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Promoter

   None

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB2B

   Plasmid

   #195284

   Purpose

   Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A Puro
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Frankenbody-FLAG-mRuby2

   Plasmid

   #175294

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with mRuby2
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. tau exon 9–12 splicing minigene

   Plasmid

   #121165

   Purpose

   Generates circular RNAs from a Tau minigene

   Depositor

   Stefan Stamm

   Article

   Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

   Insert

   Tau exons 9–12 (MAPT Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Frankenbody-FLAG-Halo

   Plasmid

   #175296

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with HaloTag
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-1 shRev-Erbalpha

   Plasmid

   #22747

   Depositor

   Bruce Spiegelman

   Article

   Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

   Insert

   shRev-Erbalpha (Nr1d1 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

8. Tn7 integration plasmid

   Plasmid

   #154134

   Purpose

   Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Inserts

   left and right end of Tn7 arm
   Spectinomycin resistant gene
   

   Expression

   Bacterial

   Promoter

   Spectinomycin resistance gene promoter and no

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Braf

   Plasmid

   #40775

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   Braf (BRAF Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Starting Met removed

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Plenti-ARE-HSVTK-luc

    Plasmid

    #161789

    Purpose

    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

    Depositor

    Hans-Guido Wendel

    Article

    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

    Inserts

    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EF1a and Murine Gclc ARE

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+) Flag-His-ATM kd

    Plasmid

    #31986

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM kd (ATM Human)
    

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    3'UTRf 9 nucl/D2870A N2875k. catalytically …

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid

    #174649

    Purpose

    Live-cell marker for stable microtubules

    Depositor

    Lukas Kapitein

    Article

    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

    Insert

    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    

    Tags

    mNeongreen-mNeongreen

    Expression

    Mammalian

    Mutation

    rigor mutation G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny, Cesar Cuevas-Velazquez

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQLink-GST-10xCTD

    Plasmid

    #138472

    Purpose

    Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    10x CTD repeat

    Promoter

    T5

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX4T1-Rpb4/7

    Plasmid

    #138484

    Purpose

    Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Inserts

    Rpb4 (POLR2D Human)
    Rpb7 (POLR2G Human)
    

    Tags

    6xHis and GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQLink-GST-4xCTD

    Plasmid

    #138471

    Purpose

    Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    4x CTD repeat

    Promoter

    T5

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFPN1-gamma-tubulin

    Plasmid

    #87956

    Purpose

    Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The protein is a sh-gamma-tubulin resistant gene …

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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