We narrowed to 9,512 results for: try

1. p3E ubb polyA

   Plasmid

   #195977

   Purpose

   Gateway p3E vector

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubb:polyA (ubb Zebrafish)
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-MBP-TEV

   Plasmid

   #105771

   Purpose

   Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   MBP-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-Runx1+23

   Plasmid

   #69602

   Purpose

   Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

   Depositor

   Leonard Zon, Owen Tamplin

   Article

   Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

   Inserts

   Runx1 +23 intronic enhancer (Runx1 Mouse)
   Mouse beta-globin minimal promoter
   

   Use

   5' gateway entry vector

   Promoter

   C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-BiFC-Venus

   Plasmid

   #105804

   Purpose

   Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   VN173 (I152L); VC155

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)_Nav1.6WT-HA

   Plasmid

   #239762

   Purpose

   Expresses mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag_HsIRE1a_pBabePuro

   Plasmid

   #54337

   Purpose

   N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

   Depositor

   David Ron

   Article

   Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

   Insert

   IRE1a (ERN1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and preprotrypsin signal sequence

   Expression

   Mammalian

   Mutation

   The endogenous IRE1a signal sequence (aa1-18) was…

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. SRC_HUMAN_D0

   Plasmid

   #79700

   Purpose

   This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SRC (SRC Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pYPQ233-STU

   Plasmid

   #138111

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   dLbCas12a-SRDX
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2xFlag CMV2-YAP2-1st&2nd WW mutant

   Plasmid

   #19048

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 199 changed to Alanine,and Proline 202…

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

    Plasmid

    #177669

    Purpose

    Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

    Depositor

    Karl Deisseroth

    Article

    Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    nEF

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC1-V16-eGFP

    Plasmid

    #122033

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    eGFP
    

    Use

    AAV

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac3-EcLeu TAG-EGFP**

    Plasmid

    #228785

    Purpose

    EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcLeuRS
    EcLtR
    EGFP**
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-neo:Beta2meGFP

    Plasmid

    #45096

    Depositor

    Henry Lester

    Article

    Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

    Insert

    nAChR beta2 (Chrnb2 Mouse)
    

    Expression

    Mammalian

    Mutation

    There are strain differences between C57BJ/6 and …

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-TEV-EGFP

    Plasmid

    #105793

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223 SARS-CoV-2 ORF3B

    Plasmid

    #141272

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pT3EF5a-PIK3CA H1047R

    Plasmid

    #180034

    Purpose

    Entry vector of PIK3CA H1047R for gateway cloning.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. DYR1A_HUMAN_D0

    Plasmid

    #79690

    Purpose

    This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DYRK1A (DYRK1A Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pKK-FLAG-BirA-TEV

    Plasmid

    #105770

    Purpose

    Expression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    FLAG-BirA-TEV

    Expression

    Mammalian

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-TEV-ProteinA

    Plasmid

    #105788

    Purpose

    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-ProteinA

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKK-mCherry-TEV

    Plasmid

    #105779

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCherry-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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