We narrowed to 18,112 results for: URE

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pCyPet-C1

Plasmid

#22779

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

CyPet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
mCherry-Septin 11 isoform II

Plasmid

#120318

Purpose

Septin 11 isoform II expression (mCherry-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-HsTRBP

Plasmid

#41096

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

TRBP (TARBP2 Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
Zfp521 full length

Plasmid

#104189

Purpose

Expression of full length Zfp521 in mammalian cells

Depositor

Kathryn Hentges

Article

AlDallal et al Leuk Res. 2016 Jul;46:10-7. doi: 10.1016

Insert

Zfp521 (Zfp521 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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H6-auxilin

Plasmid

#62571

Purpose

6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

Depositor

Ernst Ungewickell

Article

Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

Insert

Auxilin

Tags

6XHis

Expression

Bacterial

Mutation

*see comment below

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Article

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pHCMV_KUNV_PrME

Plasmid

#207285

Purpose

Mammalian expression of Kunjin virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

KUNV_PrME

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pgex-cIAP1 mut

Plasmid

#8334

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

ciap1mut (BIRC2 Human)

Tags

GST

Expression

Bacterial

Mutation

H588A

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only
R4227X-F

Plasmid

#21371

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG (following D4226)

Expression

Mammalian

Mutation

Changed R4227 to stop codon

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
B2088_BTB-IDR-FRB

Plasmid

#215101

Purpose

Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-mCherry-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-mKate2

Plasmid

#105950

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC-CAAX

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NKB

Plasmid

#184609

Purpose

For expression of a fluorescent sensor for neurokinin B in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NKB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev_O

Plasmid

#147113

Purpose

Mammalian Expression of HIV-Rev

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Septin 11 isoform II

Plasmid

#120317

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-Rsn2

Plasmid

#61686

Purpose

bacterial expression of ranaspumin 2 as a His-tagged fusion protein

Depositor

Brian Smith

Article

Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

Insert

Ranaspumin-2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-A4DARPin

Plasmid

#198288

Purpose

Produces PVCs retargeted with anti-lysozyme DARPin A4

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-A4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_PTV_GP

Plasmid

#207300

Purpose

Mammalian expression of Punta toro phlebovirus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

PTV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBS_hNFATc4nuc

Plasmid

#24586

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

NFATc4 (NFATC4 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S167A, S169A, S172A, S173A, S174A, S213A, S217A, …

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pMXs-Dppa4

Plasmid

#13362

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 4 (Dppa4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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PNMT

Plasmid

#64078

Purpose

Expression of human Phenylethanolamine N-methyltransferase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

Insert

Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only
pRK Smad3 deltaC Flag

Plasmid

#12626

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated Smad3 (aa 1-382)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
B2395_CIBN-BTB-IDR-FRB

Plasmid

#215107

Purpose

Expresses CIBN-BTB-mCherry-FUS(IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CIBN-BTB-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nup88

Plasmid

#64281

Purpose

Mammalian expression of full-length Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Tags

his and myc

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only
pSe-Rp1

Plasmid

#111644

Purpose

For construction of spacer and repeat arrays for Sulfolobus islandicus REY154A

Depositor

Qunxin She

Article

Peng et al Nucleic Acids Res. 2015 Jan;43(1):406-17

Type

Empty backbone

Use

Archaeal expression vector

Promoter

araS

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF12

Plasmid

#67113

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF12 (TCF12 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pKDR-TetOn

Plasmid

#183577

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Expression

Mammalian

Promoter

Tet-On

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-GFP

Plasmid

#184777

Purpose

Expresses H2B-GFP in human pluripotent stem cells

Depositor

Ivana Barbaric

Article

Price et al Curr Protoc. 2022 May;2(5):e435. doi: 10

Insert

H2BC11 (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc15_pvc12-HiBiT

Plasmid

#198309

Purpose

Negative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc15_pvc12-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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