We narrowed to 23,150 results for: CRISPR

1. pGEL1019

   Plasmid

   #225271

   Purpose

   Cas12i2Max variant backbone for gene editing in rice

   Depositor

   Yong Zhang

   Article

   He et al Plant Physiol. 2025 Apr 30;198(1):kiaf12

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9- AsiA_m2.1

   Plasmid

   #158065

   Purpose

   Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

   Depositor

   Harris Wang

   Article

   Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

   Insert

   dCas9-AsiA_m2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF525-AcrIIA4_gb39_lenti

   Plasmid

   #138305

   Purpose

   Lenti expression of AcrIIA4

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLsCas13a-gRNA-NT

   Plasmid

   #164857

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-NT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ultra CAST

   Plasmid

   #236111

   Purpose

   Encodes sfGFP BbsI dropout for cloning in guide RNA sequence, encodes mScarlet BsmBI/BsaI dropout for cloning in cargo sequence

   Depositor

   Jeffrey Barrick

   Article

   VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK33/Cas9

   Plasmid

   #133781

   Purpose

   Expresses drosophila-optimized Cas9

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

   Insert

   Drosophila-optimized Cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCSh5vio-2300

   Plasmid

   #170987

   Purpose

   Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300 violacein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placI

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT32

   Plasmid

   #204297

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAL-rfp_T

   Plasmid

   #74075

   Purpose

   pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA rfp (T)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid

    #174138

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHelper_ShCAST

    Plasmid

    #127922

    Purpose

    Expresses ShCAST and a crRNA targeting PSP1

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. bRA66

    Plasmid

    #100952

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. vHB16

    Plasmid

    #193455

    Purpose

    Expression of GFP-Cas9-NLS and gRNA in A. castellanii

    Depositor

    Chantal Abergel

    Article

    Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

    Insert

    tRNA-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB166

    Plasmid

    #86998

    Purpose

    CRISPR/Cas9 in fission yeast using fluoride selection

    Depositor

    Julien Berro

    Article

    Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas1+2

    Plasmid

    #72676

    Purpose

    Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

    Depositor

    Udi Qimron

    Article

    Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

    Insert

    Cas1, Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX852

    Plasmid

    #62884

    Purpose

    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAH01Sp::Cas9

    Plasmid

    #128160

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJAT77

    Plasmid

    #204295

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX854

    Plasmid

    #62886

    Purpose

    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa573-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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