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pAAV-HDR-sphys-3XHA

Plasmid

#163690

Purpose

plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

triple HA tag insertion into endogenous mouse synaptophysin gene

Use

AAV

Tags

triple HA tag

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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px330 EQR

Plasmid

#101733

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBDT

Plasmid

#62660

Purpose

Targeting vector for PITT landing pad knock-in into the Rosa26 locus

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

eGFP
neomycin

Use

Mouse Targeting

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBDU

Plasmid

#62661

Purpose

Targeting vector for PITT landing pad knock-in into the Rosa26 locus

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

eGFP
neomycin

Use

Mouse Targeting

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBGV

Plasmid

#62664

Purpose

Donor vector for mCFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

mCFP

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSR21

Plasmid

#69156

Purpose

read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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p201N 1514

Plasmid

#47025

Purpose

Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

Depositor

Wayne Parrott

Article

Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

Inserts

gma-miR1514 recognition sequence
nptII selectable marker

Use

RNAi

Promoter

GmUbi and StUbi-3

Available Since

Aug. 30, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pTW2498

Plasmid

#252581

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA6 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtCHLl1 gRNA6 and gRNA 4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pFlagTurboNTD(N3)

Plasmid

#253059

Purpose

Contains a multiple cloning site to insert a gene upstream of Flag-TurboID (N-term aa1-194)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-NTD

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pmycTurboCTD(C1)

Plasmid

#253060

Purpose

Contains a multiple cloning site to insert a gene downstream of myc-TurboID (C-term aa195-320)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-CTD

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pmycTurboCTD(N3)

Plasmid

#253061

Purpose

Contains a multiple cloning site to insert a gene upstream of myc-TurboID (C-term aa195-320)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-CTD

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pFlagTurboNTD(C1)

Plasmid

#253058

Purpose

Contains a multiple cloning site to insert a gene downstream of Flag-TurboID (N-term aa1-194)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-NTD

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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SAGA032

Plasmid

#240781

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA031

Plasmid

#240780

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA030

Plasmid

#240779

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA023

Plasmid

#240772

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA022

Plasmid

#240771

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA021

Plasmid

#240770

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA019

Plasmid

#240768

Purpose

Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP PrhaBAD BCD_medium - gfp -tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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