We narrowed to 6,189 results for: cas9 expression plasmid

1. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-TadA7.10-SpG N aa2-713-InteinN

   Plasmid

   #206967

   Purpose

   Expresses TadA7.10 and SpG cas9N by the constitutive CMV promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

   Insert

   CMV, TadA7.10, SpG N
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

   Plasmid

   #208111

   Purpose

   Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

   Insert

   SpG C, U6, scaffold
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABEmax-SpRY

   Plasmid

   #195279

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

   Depositor

   Petros Patsali, Carsten Lederer

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-ABEmax-SpG

   Plasmid

   #195278

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

   Depositor

   Petros Patsali, Carsten Lederer

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7

   Plasmid

   #220129

   Purpose

   Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   MYL2, inteinC, nSauri Cas9C
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MYL2

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

   Plasmid

   #208289

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA; mutations in iSpyMac

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

    Plasmid

    #211817

    Purpose

    AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    N-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter and U6 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

    Plasmid

    #211818

    Purpose

    AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    C-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter and U6 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZac2.1 U6-SaAi9L-U6-SaAi9R

    Plasmid

    #78604

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    gRNAs for SaCas9 targeting Ai9 locus
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN

    Plasmid

    #220128

    Purpose

    Expresses TadA8e and Sauri cas9N by MYL2 promoter, and add an HA tag fused to TadA

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, TadA, nSauri Cas9N, inteinN
    

    Use

    AAV

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

    Plasmid

    #226915

    Purpose

    Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, TadA, nSp Cas9N, inteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

    Plasmid

    #226916

    Purpose

    Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, inteinC, nSp Cas9C
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CASI-InteinC-NG C aa714-1368-U6-Lmna sgRNA1

    Plasmid

    #206974

    Purpose

    Expresses NG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    NG aa714-1368, U6, Lmna sgRNA1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1

    Plasmid

    #208110

    Purpose

    Expresses SpG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    SpG C, U6, Lmna sgRNA1, scaffold
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p426*-SpSgH

    Plasmid

    #136993

    Purpose

    Helper plasmid for SpCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p426*-SaSgH

    Plasmid

    #136994

    Purpose

    Helper plasmid for SaCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-G6-vioABE

    Plasmid

    #73438

    Purpose

    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF-LEF and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, Chicken, S. pyogenes)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PAJ389

    Plasmid

    #104147

    Purpose

    dCas9 plasmid expressing 2A sRNA trigger

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PAJ390

    Plasmid

    #104148

    Purpose

    dCas9 plasmid expressing 3A sRNA trigger

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PAJ408

    Plasmid

    #104150

    Purpose

    dCas9 plasmid expressing full length mKate2 mRNA without RBS

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAbaMCi-GFP-gfp_pJMP2768

    Plasmid

    #208889

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFTK056

    Plasmid

    #171328

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    SpCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFTK063

    Plasmid

    #171335

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    dSpCas9(m4) (for fusion)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge