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pET-45b-HOXA13(WT)-IDR-mCherry

Plasmid

#145284

Purpose

Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(WT)-IDR (HOXA13 Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13+7A-IDR

Plasmid

#145239

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13+7A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD13wt-IDR

Plasmid

#145244

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)TK-VSV

Plasmid

#139353

Purpose

Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA expressing aa 1-895 (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 896-1187

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-GPR153

Plasmid

#106053

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR153; Opto-GPR153; E7)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR153 (GPR153 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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gh17

Plasmid

#106701

Purpose

expression of gRNA targeting GALNT17

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT17 (GALNT17 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3

Plasmid

#112843

Purpose

transient/stable overexpression of the C-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

C2 domain of Catulin

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1

Plasmid

#112841

Purpose

transient/stable overexpression of the N-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

N domain of Catulin

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 Y71A

Plasmid

#71865

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Tyrosine 71 mutated to Alanine

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
KLHL31 pgk (1088)

Plasmid

#62031

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KLHL31 (Klhl31 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
Pet28a His6-HSPB7 (zebrafish) WT

Plasmid

#108917

Purpose

Express WT Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Tags

His6

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2F04

Plasmid

#70901

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mod(mdg4) (mod(mdg4) Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
ATP1A1_plasmid_donor_RD

Plasmid

#86551

Purpose

Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 (ATP1A1 Human)

Use

CRISPR and TALEN; Co-selection via hdr using ouab…

Expression

Mammalian

Mutation

Q118R, N129D

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau P301L

Plasmid

#46908

Purpose

expresses EGFP tagged Tau P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau P301L (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

P301L

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.Tau2N4R

Plasmid

#226396

Purpose

Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

His.3C

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-mito

Plasmid

#229232

Purpose

Expresses GFP localized to mitochondria

Depositor

Suzanne Pfeffer

Article

Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

Insert

EGFP-MAOA (MAOA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2364

Plasmid

#142051

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREB1 (CREB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.eGFP.2A.wtTau

Plasmid

#140424

Purpose

AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

Depositor

Bradley Hyman

Article

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau (MAPT Synthetic, Human)

Use

AAV

Tags

eGFP

Promoter

CBA

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-his-3C_Tau352

Plasmid

#100092

Purpose

Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

Depositor

Nikolai Sluchanko

Article

Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

Insert

Fetal microtubule-associated protein tau (MAPT Human)

Tags

His6

Expression

Bacterial

Mutation

wild type sequence

Promoter

T7

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSL21-VEX

Plasmid

#158230

Purpose

Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

Depositor

Junwei Shi

Article

Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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