We narrowed to 12,112 results for: LEA
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Plasmid#98183PurposeCodes for full length, GFP tagged mutant Bag3DepositorAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only
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274_pSKB3-Venus-LUMP
Plasmid#65976PurposeBacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensorDepositorInsertLUMP
TagsVenus and thrombin site (11-residue linker that h…ExpressionBacterialPromoterT7Available sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1377
Plasmid#29178PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1264
Plasmid#29136PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1602
Plasmid#29305PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle131 (MKI67 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable sinceNov. 4, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-STIM1
Plasmid#228952PurposeRetroviral expression of mCherry-STIM1DepositorAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TVBB N-term-mTurquoise2
Plasmid#169222PurposeTargeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2
UseTargeting vector backboneAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LOx-IRES-EGFP
Plasmid#166877PurposeExpress the bacterial lactate oxidase (LOx) enzyme in mammalian cellsDepositorInsertLactate oxidase
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Sox2
Plasmid#136612PurposeDox-inducible lentiviral vector expressing mouse Sox2DepositorAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 NFX1
Plasmid#67131PurposeStandard for cell-free expression benchmarking.DepositorAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
4YWQ
Plasmid#69953PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRC3H1:YTC019-B07:C232791 (RC3H1 Human)
TagsHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
NET50 pmRFP-N2 (489)
Plasmid#61983Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GLYR1
Plasmid#38946PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 DOX off blast POLE
Plasmid#241437PurposeExpresses DOX-suppressible POLEDepositorInsertPOLE (POLE Human)
Tags3xFLAGExpressionMammalianMutationSilent Mutations disrupt shRNA targeting sitesAvailable sinceJan. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHis-TEV-Nsp15
Plasmid#187656PurposeE. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tagDepositorInsertNsp15 (N Severe acute respiratory syndrome coronavirus 2)
TagsTEV protease cleavable His6-tagExpressionBacterialMutationnonePromoterT7lacAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
Plasmid#181956PurposeGenetically encoded FRET-based cAMP indicator fused to AKAP79.DepositorInsertAKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
TagsAKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…ExpressionMammalianPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
Tags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDD-pcDNA3.1(+)
Plasmid#172313PurposeExpresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
Tags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE3334
Plasmid#107537PurposeExpresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.DepositorInsertsMb crRNA
hMbCpf1(RVR mutant)
Tags3xHA and NLSExpressionMammalianMutationN576R, K582V, N586RPromoterCMV and human U6Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only