We narrowed to 17,706 results for: jun

1. pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

   Plasmid

   #178999

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-sg-CMV152

   Plasmid

   #179916

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-sg-CMV51

   Plasmid

   #179915

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-sg-CMV162

   Plasmid

   #179917

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-sg-mGreenLantern332

   Plasmid

   #179914

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX552-rScn2a-3xV5-Syn-miRFP670

   Plasmid

   #182566

   Purpose

   For rat Nav1.2 knockin with 3xV5 at the extracellular loop

   Depositor

   James Zhe Liu

   Article

   Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

   Inserts

   rat Scn2a gRNA and 3xV5 donor DNA
   miRFP670
   

   Use

   AAV and CRISPR

   Promoter

   Syn and U6

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmGW182-GW10_338_438A_D

   Plasmid

   #146162

   Purpose

   Insect Expression of DmGW182-GW10_338_438A

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

   Insert

   DmGW182-GW10_338_438A (gw Fly)
   

   Expression

   Insect

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-lambdaN-HA-DmGW182-GWG10A_D

   Plasmid

   #146163

   Purpose

   Insect Expression of DmGW182-GWG10A

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

   Insert

   DmGW182-GWG10A (gw Fly)
   

   Expression

   Insect

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-HA-DmGW182-WG338_438A_D

   Plasmid

   #146164

   Purpose

   Insect Expression of DmGW182-WG338_438A

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

   Insert

   DmGW182-WG338_438A (gw Fly)
   

   Expression

   Insect

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneo-lambdaN-HA-HsAGO1_C

    Plasmid

    #146083

    Purpose

    Mammalian Expression of HsAGO1

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    HsAGO1 (AGO1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG.mCherry-INBox-eDHFR

    Plasmid

    #176202

    Purpose

    Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    mCherry-INBox-eDHFR
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG.MCAK-mCherry-eDHFR

    Plasmid

    #176204

    Purpose

    MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    MCAK-mCherry-eDHFR
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-EGFP-PML peptide

    Plasmid

    #175247

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    PML (PML Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH858

    Plasmid

    #172176

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
    

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJH859

    Plasmid

    #172177

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
    

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH580

    Plasmid

    #172164

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 3f2v2+B); AraC
    

    Expression

    Bacterial

    Mutation

    PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH581

    Plasmid

    #172165

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 3f2+C); AraC
    

    Expression

    Bacterial

    Mutation

    PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH750

    Plasmid

    #172170

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.3f2, deleted sumo
    

    Expression

    Bacterial

    Mutation

    Evolved PylBCD operon; SUMO tag deleted from PylB…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH751

    Plasmid

    #172171

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.JM10.1, deleted sumo
    

    Expression

    Bacterial

    Mutation

    Evolved PylBCD operon; SUMO tag deleted from PylB…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJH768v2

    Plasmid

    #172173

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.3f2]
    

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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