We narrowed to 253 results for: T7 rna polymerase

1. pSC101-pT7-T3RNAP

   Plasmid

   #140872

   Purpose

   T7 promoter driven synthesis of T3 RNA polymerase

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   T3 RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

   Plasmid

   #228508

   Purpose

   Bacterial Hybrid Driver Plasmid

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   T7 RNAP, LARP-I5CHO (#1)
   

   Use

   Synthetic Biology

   Tags

   Umu Degron, GTG-start codon

   Expression

   Mutation

   R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7RNAP Cyslight

   Plasmid

   #205077

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. T7RNAP Cyslight V174C

   Plasmid

   #205078

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S V174C

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE-WT-RGVG-M6

   Plasmid

   #70172

   Purpose

   Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

   Depositor

   Andrew Ellington

   Article

   Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

   Insert

   RGVG-M6 T7 RNA polymerase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T5/Lac

   Available since

   Dec. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEcRNAP6

   Plasmid

   #128940

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoA (rpoA E. coli)
   RpoB
   RpoC-ppx(His)10
   RpoZ
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   PreScission protease site (GE Healthcare; LEVLFQG…

   Promoter

   T7

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-T7pol

   Plasmid

   #59926

   Purpose

   Expression vector for T7 RNA polymerase gene

   Depositor

   Ian Wickersham

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEcRNAP5

   Plasmid

   #128939

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoAppx234-241-(His)10 (rpoA Escherichia coli)
   RpoB
   RpoC
   RpoZ
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 234-241 are substituted for a PreScissio…

   Promoter

   T7

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

   Plasmid

   #217808

   Purpose

   Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-His-T7RNAP

    Plasmid

    #124138

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    T7 RNA polymerase (T7p07 Enterobacteria phage T7)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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