Addgene

We narrowed to 3,512 results for: AAT

Showing: 41 - 80 of 3512 results

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sgAAT Nicking

Plasmid

#169842

Purpose

Express a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutation

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

sgAAT Nicking (SERPINA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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III-AAAT

Plasmid

#43478

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-AAAT

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
III-AATA

Plasmid

#43487

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-AATA

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
III-AATC

Plasmid

#43488

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-AATC

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
III-AATG

Plasmid

#43489

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-AATG

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
III-AATT

Plasmid

#43490

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-AATT

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
III-TAAT

Plasmid

#43670

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-TAAT

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
III-GAAT

Plasmid

#43606

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-GAAT

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
III-CAAT

Plasmid

#43542

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

III-CAAT

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pAGT8163 (AATG_ttLbCas12a-i_TTCG)

Plasmid

#192453

Purpose

intronized ttLbCas12a for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Tags

Expression

Mutation

temperature-tolerant mutation (D156R)

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-hAAT-W

Plasmid

#126704

Purpose

Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

hAAT (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVp

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1alphaL-hAAT-W

Plasmid

#24527

Purpose

Lentiviral overexpression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

alpha-1 antitrypsin (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pNH605-CCAAT-YFP

Plasmid

#172132

Purpose

Fluorescent reporter for HAP complex activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xCCAAT-YFP

Use

Vector for genomic integration into the yeast gen…

Tags

Expression

Yeast

Mutation

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAGT8162 (AATG_dttLbCas12a-i_TTCG)

Plasmid

#192350

Purpose

L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

AATG_dttLbCas12a-i_TTCG

Use

CRISPR

Tags

Expression

Mutation

D156R; E925Q

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pegAAT K342E correction

Plasmid

#169841

Purpose

Express a pegRNA used for correction (via A•T-to-G•C) of the E342K mutation

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

pegAAT K342E correction (SERPINA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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AATK gRNA (BRDN0001146583)

Plasmid

#77315

Purpose

3rd generation lentiviral gRNA plasmid targeting human AATK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AATK (AATK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AATK gRNA (BRDN0001147695)

Plasmid

#77316

Purpose

3rd generation lentiviral gRNA plasmid targeting human AATK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AATK (AATK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AATK gRNA (BRDN0001148887)

Plasmid

#77314

Purpose

3rd generation lentiviral gRNA plasmid targeting human AATK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AATK (AATK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-EGFP-mouse Plaat3 (WT)

Plasmid

#167987

Purpose

Expresses EGFP-Plaat3 in mammalian cells

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls3 (Plaat3 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag

Plasmid

#189639

Purpose

sTALED for plants

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a-DAAT-V33G/T242G

Plasmid

#159278

Purpose

Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

Depositor

Roberto Chica

Article

Parmeggiani et al ACS Catalysis 9, 3482–3486.

Insert

Engineered d-alanine aminotransferase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Valine 33 and threonine 242 have been mutated to …

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Tags

Expression

Mutation

V30M

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti CGIP + Z-AAT target

Plasmid

#86007

Purpose

lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
Z-AAT target

Use

Lentiviral

Tags

Expression

Mutation

V30M

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-UBC-hAAT-IRES-ZsGreen-W

Plasmid

#126702

Purpose

lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Insert

Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UBC

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-EGFP-mouse Plaat3 (N157stop)

Plasmid

#168771

Purpose

Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls3 (Plaat3 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

Plasmid

#168772

Purpose

Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls (plaat1 Zebrafish)

Use

Tags

EGFP

Expression

Mutation

Promoter

alphaA-crystallin promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

Plasmid

#163030

Purpose

Expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Mutation

Promoter

Alb-AAT

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

Plasmid

#163025

Purpose

Expression of CRISPRi with gRNA against mouse Pcsk9

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Mutation

Promoter

Alb-AAT

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397N

Plasmid

#189647

Purpose

psa A Right _G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 H245N (AAT)

Plasmid

#212551

Purpose

Encodes SARS-CoV-2 spike H245N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike H245N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

H245N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 D950N (AAT)

Plasmid

#212425

Purpose

Encodes SARS-CoV-2 spike D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D950N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D950N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 D936N (AAT)

Plasmid

#212405

Purpose

Encodes SARS-CoV-2 spike D936N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D936N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D936N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 T20N (AAT)

Plasmid

#212359

Purpose

Encodes SARS-CoV-2 spike T20N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T20N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

T20N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT)

Plasmid

#212347

Purpose

Encodes SARS-CoV-2 spike K417N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

K417N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE

Plasmid

#189643

Purpose

16S rRNA Right_G1397C_ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397N

Plasmid

#189644

Purpose

16S rRNA Right_G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397C_ABE

Plasmid

#189648

Purpose

psa A Right _G1397C_ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 N

Plasmid

#189651

Purpose

rbc L 294 Right_G1397 N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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