We narrowed to 770 results for: NEB

1. pMalAnPL

   Plasmid

   #67281

   Purpose

   Expresses MBP-Anacystis nidulans photolyase in E. coli

   Depositor

   Aziz Sancar

   Insert

   photolyase Anacystis nidulans
   

   Use

   Tags

   maltose binding protein

   Expression

   Bacterial

   Mutation

   none known

   Promoter

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MT1K_K20T

   Plasmid

   #105708

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 1k subisoform with Thr at positon 20

   Depositor

   Artur Krężel

   Insert

   Metallothionein 1k
   

   Use

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   This subisoform has a K20T substitution

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MT1B

   Plasmid

   #105701

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 1b

   Depositor

   Artur Krężel

   Insert

   Metallothionein 1b (MT1B Human)
   

   Use

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMal-T-Avi-His/BirA

   Plasmid

   #102962

   Purpose

   Vector for in vivo biotinylation of a fusion protein in bacteria.

   Depositor

   Tonia Rex

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmal_c5x_RNAse_Inhib

   Plasmid

   #153314

   Purpose

   Bacterial expression of an RNase inhibitor

   Depositor

   Drew Endy, Philippa Marrack

   Insert

   RNAse Inhibitor
   

   Use

   Tags

   Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pHN764

   Plasmid

   #178819

   Purpose

   Expression of EndoH in E. coli

   Depositor

   Kazuhiro Abe

   Insert

   synthetic gene encoding endo-β-N-acetylglucosaminidase H
   

   Use

   Tags

   HIs8 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET11a-p65(19-325)

   Plasmid

   #44744

   Depositor

   Gourisankar Ghosh

   Insert

   p65 (Rela Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AA 19-325

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pXylP-HygR

   Plasmid

   #168131

   Purpose

   Expression of HygR from the xylose-inducible xylP promoter

   Depositor

   Michael Bromley

   Insert

   XylP-HygR
   

   Use

   Aspergillus expression

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   xylP

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MT4

   Plasmid

   #105711

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 4

   Depositor

   Artur Krężel

   Insert

   Metallothionein 4 (MT4 Human)
   

   Use

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDY0551-Luciferase RADARS

    Plasmid

    #182538

    Purpose

    Mammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Insert

    RADARS Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MBP-Benzonase

    Plasmid

    #218192

    Purpose

    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

    Depositor

    Cheryl Arrowsmith

    Insert

    nucA
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. MT1A

    Plasmid

    #105700

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1a

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1a (MT1A Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MT1H

    Plasmid

    #105706

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1h

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1h (MT1H Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYZ205

    Plasmid

    #104586

    Purpose

    Express SNAP-6His-CBD

    Depositor

    Yinhua Zhang, New England Biolabs

    Insert

    SNAP-6His-CBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTXB3-Flag PLK1

    Plasmid

    #68271

    Purpose

    bacterial expression of Plk1

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET11a-p52(1-398)

    Plasmid

    #44733

    Depositor

    Gourisankar Ghosh

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AA 1-398

    Promoter

    T7

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

17. MT1G_isoform2

    Plasmid

    #105705

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1g subisoform 2 with deletion of Alanine 10

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1g (MT1G Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    This subisoform has a deletion of alanine 10

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MT4_C30Y R31W

    Plasmid

    #105712

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution

    Depositor

    Artur Krężel

    Insert

    Metallothionein 4 (MT4 Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    This subisoform has a C30Y and R31W substitution

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTXB3-Flag PLK1 T210D

    Plasmid

    #68272

    Purpose

    bacterial expression of Plk1 T210D mutant

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    T210D

    Promoter

    T7

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. HOXB7-pMAL

    Plasmid

    #20996

    Depositor

    Corey Largman

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Tags

    mal

    Expression

    Bacterial

    Mutation

    Coding region only

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

21. GPD3_KW

    Plasmid

    #224151

    Purpose

    IPTG-inducible expression of C. elegans protein GPD-3 with a chitin tag

    Depositor

    Katherine Walstrom

    Insert

    GPD-3 (gpd-3 Nematode)
    

    Use

    Tags

    intein-CBD

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GPD1_SB

    Plasmid

    #224147

    Purpose

    IPTG-inducible expression of C. elegans protein GPD-1 with a chitin tag

    Depositor

    Katherine Walstrom

    Insert

    GPD-1 (gpd-1 Nematode)
    

    Use

    Tags

    Ala-intein-CBD

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ADH1_KW

    Plasmid

    #224149

    Purpose

    IPTG-inducible expression of C. elegans protein ADH-1 with a chitin tag

    Depositor

    Katherine Walstrom

    Insert

    ADH-1 (sodh-1 Nematode)
    

    Use

    Tags

    Ala-intein-CBD

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTYB2-eIF1

    Plasmid

    #37217

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pTYB2-eIF5

    Plasmid

    #37219

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pTYB2-eIF1A

    Plasmid

    #37218

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF1A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pCM62

    Plasmid

    #45826

    Depositor

    Mary Lidstrom

    Article

    Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

    Type

    Empty backbone

    Use

    Broad host range cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOpen-GBDpol (exo)

    Plasmid

    #165572

    Purpose

    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pCVD27

    Plasmid

    #63690

    Purpose

    540bp ctxA gene probe EcoRI linkered

    Depositor

    James Kaper

    Article

    Kaper et al DNA probes for infectious disease. CRC P

    Insert

    ctxA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pCW4

    Plasmid

    #8484

    Depositor

    Nancy Craig

    Article

    Waddell et al Genes Dev 1988 Feb;2(2):137-49.

    Insert

    Tn7ABCDE (tnsA Bacteria)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCW15

    Plasmid

    #8485

    Depositor

    Nancy Craig

    Article

    Waddell et al Genes Dev 1988 Feb;2(2):137-49.

    Insert

    Tn7ABC (tnsA Bacteria)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTXB1-sfGFP

    Plasmid

    #51562

    Purpose

    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    superfolder GFP
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pU1_000

    Plasmid

    #186773

    Purpose

    * Engineered U1 snRNA plasmid for insertion of guide sequences.

    Depositor

    Eugene Yeo

    Article

    Hatch et al Methods. 2022 Jun 25;205:140-148. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U1 Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pmVirD2

    Plasmid

    #90314

    Purpose

    mVirD2 expression plasmid

    Depositor

    Bjorn Hogberg

    Article

    Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

    Insert

    A. tumefaciens VirD2 from aa 1 to 204
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7/T5 IPTG inducible

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMal-E6LLO

    Plasmid

    #51281

    Purpose

    Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria

    Depositor

    Dane Wittrup

    Article

    Pirie et al Mol Cancer Ther. 2013 Sep;12(9):1774-82.

    Insert

    E6LLO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTXB1-FIS

    Plasmid

    #51564

    Purpose

    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    FIS
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTXB1-CII

    Plasmid

    #51566

    Purpose

    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    cII
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pmVirD2-GFP

    Plasmid

    #90315

    Purpose

    mVirD2-GFP expression plasmid

    Depositor

    Bjorn Hogberg

    Article

    Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

    Insert

    A. tumefaciens VirD2 from aa 1 to 204 - GFP
    

    Use

    Tags

    6xHis and GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7/T5 IPTG inducible

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBW-m4092677

    Plasmid

    #153883

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wt;handful of SNPs as watermarks

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pTXB1-CI

    Plasmid

    #51565

    Purpose

    Expression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    cI
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge