We narrowed to 7,808 results for: 199

1. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

   Plasmid

   #199604

   Purpose

   Expression of cell permeable spCas9 in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   Cas9-T6N
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p35S:Citrine-TurboID-3xHA

   Plasmid

   #199243

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID
   

   Use

   Tags

   3xHA and TurboID-3xHA

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBQ:Citrine-TurboID-3xMyc

   Plasmid

   #199244

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID-3xMyc
   

   Use

   Tags

   TurboID-3xMyc

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis UBQ10 promoter with intron

   Available since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG_IGF1R_EGFP

   Plasmid

   #199628

   Purpose

   FRET donor for IGF1R sensor

   Depositor

   Ryohei Yasuda

   Article

   Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. BB44_pmc.DonorR5.TS

   Plasmid

   #199223

   Purpose

   Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
   

   Use

   Gene targeting donor plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1 gene promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AB65_pCAG.Cas9-D10A.rBGpA

   Plasmid

   #199256

   Purpose

   Expression construct encoding the SpCas9-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SpCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-C-STAT1-mGFP-P2A-Puro

   Plasmid

   #199345

   Purpose

   express murine STAT1 protein fused with mGFP

   Depositor

   Massimo Mallardo

   Article

   Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

   Insert

   signal transducer and activator of transcription 1 (Stat1 Mouse)
   

   Use

   Lentiviral

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

   Plasmid

   #199777

   Purpose

   enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R297T for reduced recombinase efficiency.

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-eSpCas9_sgAAVS1

   Plasmid

   #199213

   Purpose

   All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   AAVS1-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA14-pHR-SFFV-GFP-RPA43

    Plasmid

    #199445

    Purpose

    Expression of GFP-RPA43 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    RPA43 (POLR1F Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAKgfplux3

    Plasmid

    #199304

    Purpose

    Fluorescence and bioluminescence expression in Gram-negative bacteria

    Depositor

    Attila Karsi

    Article

    Ozdemir et al Pathogens. 2023 May 2;12(5):670. doi: 10

    Insert

    Chloramphenicol acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LPUtopia-7

    Plasmid

    #199212

    Purpose

    Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Inserts

    aminoglycoside phosphotransferase,
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and thymidine kinase promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA12-pHR-SFFV-BFP-B23

    Plasmid

    #199443

    Purpose

    Expression of BFP-B23 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    B23 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP

    Plasmid

    #199456

    Purpose

    Expression of stdPCP-PH in mammalian cells

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    stdPCP-PH
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA13-pHR-SFFV-HaloTag-FBL

    Plasmid

    #199444

    Purpose

    Expression of HaloTag-FBL in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    FBL (FBL Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

    Plasmid

    #199262

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

    Plasmid

    #199442

    Purpose

    CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    HITI donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDSP31

    Plasmid

    #199373

    Purpose

    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. GABA(A)R, Alpha1 scFv [N95/35]

    Plasmid

    #199430

    Purpose

    Mammalian expression of GABA(A)R, Alpha1 scFv. Derived from hybridoma N95/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingGABA(A)R, Alpha1 (Human) (GABRA1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Neurexin-1-Beta scFv [N170A/26]

    Plasmid

    #199425

    Purpose

    Mammalian expression of Neurexin-1-Beta scFv. Derived from hybridoma N170A/26.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingNeurexin-1-Beta (Human) (NRXN1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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