1. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pX458_sgRNA_Ago1_5

   Plasmid

   #172470

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX458_sgRNA_Ago1_6

   Plasmid

   #172471

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1.

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMGS7 (AAVS1sgRNA)

   Plasmid

   #126582

   Purpose

   A CRISPR/Cas9 construct to target the AAVS1 locus in humans

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AAVS1 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

   Plasmid

   #107858

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 2) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCm7RNA6A

   Plasmid

   #84367

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2 m7-gRNA6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

7. mCBFKOgRNA5

   Plasmid

   #84366

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2-gRNA5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

8. mCBFKOgRNA2

   Plasmid

   #84365

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2-gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

9. PX458_AHR_1

   Plasmid

   #101076

   Purpose

   Encodes gRNA for 3' target of human AHR

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against AHR (AHR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PX458_AHR_2

    Plasmid

    #101077

    Purpose

    Encodes gRNA for 3' target of human AHR

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against AHR (AHR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 5xtetO (tet3) Gene Desert

    Plasmid

    #131339

    Purpose

    gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Desert-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EHMT1_exon 3_gRNA

    Plasmid

    #228809

    Purpose

    CRISPR targeting of human EHMT1

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    gRNA targeting EHMT1 exon 3 (EHMT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 5xtetO HoxC 3'

    Plasmid

    #131340

    Purpose

    gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    HoxC-downstream-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EZH2-Promoter-gRNA

    Plasmid

    #131329

    Purpose

    gRNA to generate endogenously tagged -tetR-EZH2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EZH2-Promoter-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EED-F97A

    Plasmid

    #131326

    Purpose

    gRNA to generate F97A cage-mutant EED

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EED-KO-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EED-KO-gRNA-2

    Plasmid

    #131322

    Purpose

    gRNA to knockout EED. Use with EED-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EED-KO-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

17. gh23

    Plasmid

    #106696

    Purpose

    expression of gRNA targeting POMT1, Cas9, and GFP

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    POMT1 (POMT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX458-ECFP

    Plasmid

    #112220

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    ECFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U6GRNA

    Plasmid

    #68370

    Purpose

    gRNA scaffold with hU6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-SpCas9-GFP-U6-gRNA

    Plasmid

    #79144

    Purpose

    All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

    Depositor

    Jizhong Zou

    Article

    Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge