We narrowed to 107 results for: 62988

1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Publication

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   1761 Articles

   

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These results may also be relevant.

1. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Publication

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. px459-TDP-43 sgRNA1

   Plasmid

   #133762

   Purpose

   Expresses Cas9 and human TDP-43 sgRNA

   Depositor

   Shawn Ferguson

   Publication

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX459v3

   Plasmid

   #178799

   Purpose

   SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Publication

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Publication

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459-TDP 43 sgRNA2

   Plasmid

   #133763

   Purpose

   Expresses Cas9 and human TDP-43 sgRNA

   Depositor

   Shawn Ferguson

   Publication

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. B2M-SDMutation-gRNA

   Plasmid

   #215547

   Purpose

   gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

   Depositor

   Kai Wang

   Publication

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   B2M (B2M Human)
   

   Use

   CRISPR

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Publication

   Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Expression

   Mammalian

   Promoter

   u6

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Publication

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPH2A.X-GS3

   Plasmid

   #204745

   Purpose

   Expression of Cas9 and human H2AX

   Depositor

   Yuichiro Miyaoka

   Publication

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   H2AX (H2AX Human)
   

   Expression

   Mammalian

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SOX17-3'gRNA

    Plasmid

    #210465

    Purpose

    gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Publication

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    SOX17 (SOX17 Human)
    

    Use

    CRISPR

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-CTNNB1-ATG

    Plasmid

    #153429

    Purpose

    Expresses a gRNA that overlaps the startcodon of human CTNNB1

    Depositor

    Renee van Amerongen

    Publication

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    CTNNB1 gRNA (CTNNB1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPH2A.X_S139A-GS3

    Plasmid

    #204749

    Purpose

    Expression of Cas9 and human H2AX with the S139A mutation

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    H2AX S139A (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the S139A mutation

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro-C9orf72

    Plasmid

    #83439

    Purpose

    Expresses Cas9 and a gRNA targeting C9orf72

    Depositor

    Shawn Ferguson

    Publication

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human C9orf72 (C9orf72 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-sgRNA2-RPB1-N-term

    Plasmid

    #195111

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Publication

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PECAM1-3'gRNA

    Plasmid

    #235606

    Purpose

    gRNA targeting PECAM1 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Publication

    Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    PECAM1 gRNA (PECAM1 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Publication

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Publication

    Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX459-CTNNB1-S45

    Plasmid

    #164587

    Purpose

    Expresses a gRNA that overlaps the S45 codon of human CTNNB1

    Depositor

    Renee van Amerongen

    Publication

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    CTNNB1 gRNA (CTNNB1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SOX2-3'gRNA

    Plasmid

    #215543

    Purpose

    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124287

    Purpose

    sgRNA against human Col7A1

    Depositor

    Katalin Josvay, Csaba Vizler

    Has service

    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only