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We narrowed to 46 results for: Geobacillus

Showing: 21 - 40 of 46 results

  1. pKAR2-Br512g3.1

    Plasmid
    #171200
    Purpose
    Expresses Br512g3.1 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171200-plasmid-map-sequence-id-339557

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  2. pKAR2-Br512g3.2

    Plasmid
    #171201
    Purpose
    Expresses Br512g3.2 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171201-plasmid-map-sequence-id-339558

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  5. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  6. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Mutation
    Promoter
    T7, tetPA
    Available Since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    161875-plasmid-map-sequence-id-316881

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  15. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid
    #58711
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Depositor
    Hermann Gaub
    Article
    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
    Insert
    Xyl-DocI
    Use
    Tags
    HIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cysteine in Xylanase Doma…
    Promoter
    T7
    Available Since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58711-plasmid-map-sequence-id-90776

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  16. enDR3-TEV-mEGFP-NLS (pRS1451)

    Plasmid
    #240546
    Purpose
    Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.
    Depositor
    Roman Szczęsny
    Article
    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    enDR3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L52M
    Promoter
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240546-plasmid-map-sequence-id-481872

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  17. nb-enDR3-TEV-mEGFP-NLS (pRS1458)

    Plasmid
    #240547
    Purpose
    Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.
    Depositor
    Roman Szczęsny
    Article
    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    nb-enDR3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S48A, K50A
    Promoter
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240547-plasmid-map-sequence-id-481875

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  19. EcNGsC-S308P-H309D-pKK223

    Plasmid
    #131378
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera S308P H309D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available Since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131378-plasmid-map-sequence-id-259242

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  20. EcNGsC-F307A-S308A-pKK223

    Plasmid
    #131379
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera F307A S308A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are fro…
    Promoter
    tac
    Available Since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131379-plasmid-map-sequence-id-259243

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Showing: 21 - 40 of 46 results