We narrowed to 46 results for: Geobacillus

1. pKAR2-Br512g3.1

   Plasmid

   #171200

   Purpose

   Expresses Br512g3.1 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al medRxiv 2022.08.09.22278605

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (vHP47…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKAR2-Br512g3.2

   Plasmid

   #171201

   Purpose

   Expresses Br512g3.2 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al medRxiv 2022.08.09.22278605

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (vHP47…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a(+)-FPPS

   Plasmid

   #108952

   Purpose

   heterologous expression of FPPS of G. stearothermophilus in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Farnesyldiphosphat synthase (FPPS)
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. S7SZ03

   Plasmid

   #163199

   Purpose

   Inducible expression of UniProt identifier S7SZ03

   Depositor

   Martin Engqvist

   Article

   Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

   Insert

   S7SZ03
   

   Tags

   10xHis and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pThermoCas9i_ctrl

   Plasmid

   #100982

   Purpose

   Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

   Depositor

   John van der Oost

   Article

   Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

   Inserts

   Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
   ThermoCas9 single guide RNA expressing module
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   changed aspartate 8 to alanine and histidine 582 …

   Promoter

   B. coagulans DSM 1 pta promoter and B. smithii xy…

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKAR2-Br512

   Plasmid

   #161875

   Purpose

   Expresses Br512 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Maranhao et al medRxiv

   Insert

   Modified DNA Polymerase I Large Fragment (Br512)
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7, tetPA

   Available Since

   Nov. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSL4164 (pEffector_IscB,ωRNA)

   Plasmid

   #210874

   Purpose

   Expresses GstIscB and native ωRNA from constitutive promoters

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   GstIscB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL4369 (pEffector_TnpB2,ωRNA)

   Plasmid

   #210871

   Purpose

   Expresses GstTnpB2 and native ωRNA from constitutive promoters

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   GstTnpB2
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL4672 (pEffector_TnpB3,ωRNA)

   Plasmid

   #210872

   Purpose

   Expresses GstTnpB3 and native ωRNA from constitutive promoters

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   GstTnpB3
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL4673 (pEffector_TnpB4,ωRNA)

    Plasmid

    #210873

    Purpose

    Expresses GstTnpB4 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB4
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a(+)-Bst LF-6xHis

    Plasmid

    #159148

    Purpose

    expresses His-tagged large fragment of Bst polymerase

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Insert

    large fragment of Geobacillus stearothermophilus DNA polymerase I
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAtetO_6xHis-Bst-LF

    Plasmid

    #145799

    Purpose

    Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

    Depositor

    Andrew Ellington

    Article

    Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

    Insert

    Bst-LF
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    tet PA

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pIntegrase_7

    Plasmid

    #60578

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_7
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. enDR3-TEV-mEGFP-NLS (pRS1451)

    Plasmid

    #240546

    Purpose

    Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.

    Depositor

    Roman Szczęsny

    Article

    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    enDR3
    

    Expression

    Mammalian

    Mutation

    L52M

    Available Since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. nb-enDR3-TEV-mEGFP-NLS (pRS1458)

    Plasmid

    #240547

    Purpose

    Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.

    Depositor

    Roman Szczęsny

    Article

    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    nb-enDR3
    

    Expression

    Mammalian

    Mutation

    S48A, K50A

    Available Since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EcNGsC-MutY-pKK223

    Plasmid

    #131376

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera
    

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EcNGsC-S308P-H309D-pKK223

    Plasmid

    #131378

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera S308P H309D
    

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EcNGsC-F307A-S308A-pKK223

    Plasmid

    #131379

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera F307A S308A
    

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are fro…

    Promoter

    tac

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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