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CMV_dCas9-AsLOV2x3-NLSx3

Plasmid

#231567

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKM596-AePHOT-LOV2

Plasmid

#220114

Purpose

The expression of MBP fused to AePHOT-LOV2 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-LOV2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-MpPHOT-LOV2

Plasmid

#220116

Purpose

The expression of MBP fused to MpPHOT-LOV2 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-LOV2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEB1N-mApple-LZ-LOV2

Plasmid

#197418

Purpose

Mammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2022 Mar 14;32(5):1197-1205.e

Insert

MAPRE1 (MAPRE1 Human)

Use

Tags

A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_AsPT1-LOV2-EcSsra

Plasmid

#131217

Purpose

Expresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AsPT1-LOV2-EcSsra

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_AsPT1-LOV2-pep

Plasmid

#131215

Purpose

Expresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AsPT1-LOV2-pep

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS5c-mCherry-AsLOV2*(543)

Plasmid

#202712

Purpose

Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Insert

mCherry-AsLOV2*(543)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416L

Plasmid

#81036

Purpose

mammalian expression of LOV V416L, a mutant with super long relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416L

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416L

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427T

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe

Plasmid

#170623

Purpose

For the mammalian expression of mLOV2GIVe

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427V

Plasmid

#81044

Purpose

mammalian expression of LOV2 I427V

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427V

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427V

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-AG(Y63L)-LOV2

Plasmid

#178876

Purpose

Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen(Y63L)-LOV2

Use

Tags

Expression

Mammalian

Mutation

AzamiGreen: Y63L

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 C450A

Plasmid

#81031

Purpose

Mammalian expression of the dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (FA)

Plasmid

#170626

Purpose

For the mammalian expression of mLOV2GIVe GEF-deficient mutation F1685A

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (FA)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-MontiRed-LOV2

Plasmid

#178872

Purpose

Mammalian expression of PB1-MontiRed-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416T

Plasmid

#81040

Purpose

mammalian expression of LOV V416T

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2 V416T

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416T

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-MontiRed-LOV2dark

Plasmid

#178874

Purpose

Mammalian expression of PB1-MontiRed-LOV2dark

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2dark

Use

Tags

Expression

Mammalian

Mutation

LOV2: C450A, L514K, G528A, L531E, N538E

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-LOV2-OMP25

Plasmid

#233346

Purpose

Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted and N538E

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-LOV2-TMEM106B

Plasmid

#233347

Purpose

Stable expression of EGFP-LOV2(G528A, N538E)-TMEM106B in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
TMEM106B (TMEM106B Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted (TMEM106B 90-274) and N538E

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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