We narrowed to 143 results for: PCR2

1. pCR2.1-Ins2-842

   Plasmid

   #53969

   Purpose

   842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

   Depositor

   Kevin Ferreri

   Article

   Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

   Insert

   mouse Ins2 gene partial (Ins2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR2.1 Clover-LMNA Donor

   Plasmid

   #122508

   Purpose

   Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
   

   Use

   Homology repair template plasmid (donor plasmid)

   Tags

   Clover

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-pcr1P-natMX6-pcr2P

   Plasmid

   #80620

   Purpose

   to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

   Depositor

   Miguel Godinho Ferreira

   Article

   Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

   Inserts

   PCR1 from mat1-P
   PCR2 from mat1-P
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-pcr1M-natMX6-pcr2M

   Plasmid

   #80621

   Purpose

   to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

   Depositor

   Miguel Godinho Ferreira

   Article

   Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

   Inserts

   PCR1 from mat1-M
   PCR2 from mat1-M
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TC9pAB

   Plasmid

   #71310

   Purpose

   used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9
   

   Use

   In vitro transcription

   Tags

   NLS

   Expression

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TC9nickpAB

   Plasmid

   #71311

   Purpose

   used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9 nickase
   

   Use

   In vitro transcription

   Tags

   NLS

   Expression

   Mutation

   D10A nickase

   Promoter

   T7

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GPR12-DuET

   Plasmid

   #213260

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR12 (GPR12 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CYSLTR2-Tango

   Plasmid

   #66267

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CYSLTR2 (CYSLTR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. CYSLTR2-DuET

   Plasmid

   #213226

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CYSLTR2 (CYSLTR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LPAR1-DuET

    Plasmid

    #213329

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    LPAR1 (LPAR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LHP1034 - ECFP-Nterm

    Plasmid

    #32436

    Depositor

    Linda Hicke

    Insert

    ECFP
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

12. LHP1032 - EYFP-Nterm

    Plasmid

    #32438

    Depositor

    Linda Hicke

    Insert

    EYFP
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. LHP1033 - EYFP-Cterm

    Plasmid

    #32437

    Depositor

    Linda Hicke

    Insert

    EYFP
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. LHP1035 - ECFP-Cterm

    Plasmid

    #32435

    Depositor

    Linda Hicke

    Insert

    ECFP
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pHMR1B

    Plasmid

    #102958

    Purpose

    gene cloning of MR1B

    Depositor

    Gennaro De Libero, Lucia Mori

    Insert

    MR1B cDNA (MR1P1 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Stop codon has been removed by HindIII site

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSaGuide

    Plasmid

    #64710

    Purpose

    Cloning and expression of Staphylococcus aureus guide RNA

    Depositor

    Kiran Musunuru

    Insert

    Staphylococcus aureus guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAMP no 3'UTR

    Plasmid

    #129561

    Purpose

    cytomplasmic marker without terminator (less integration)

    Depositor

    Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Clim-DC1-mCherry-H2B-Venus

    Plasmid

    #129559

    Purpose

    nuclear marker

    Depositor

    Inaki Ruiz-Trillo

    Insert

    mcherry and Histone 2B fused to venus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS-1 magA

    Plasmid

    #21751

    Depositor

    Elliot Meyerowitz

    Article

    Bertani et al Gene. 2001 Feb 21. 264(2):257-63.

    Insert

    magA (magA M. magnetotacticum)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pTK565

    Plasmid

    #114690

    Purpose

    SNAP-tagging at DHC's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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