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pPROBE-gfp[tagless]

Plasmid

#40164

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PLAGL1

Plasmid

#61542

Purpose

Expresses PLAGL1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

PLAGL1 (PLAGL1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pEU3-NII-FLAGLICNot

Plasmid

#140184

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

FLAG

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-PLAGL2

Plasmid

#133380

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-PLAGL2

Plasmid

#133369

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CpcBfull-NaGLS fusion_CpcA

Plasmid

#74004

Purpose

Expresses the Nicotiana attenuata GLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

NaGLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'-deltaGL

Plasmid

#216924

Purpose

Mega' with knockout of GvpG and GvpL

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpG and GvpL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-DAGLA

Plasmid

#221289

Purpose

Expression of DAGLA (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

DAGLA (pLxIS) (Dagla Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-ORF68 (tagless)

Plasmid

#166025

Purpose

Expresses ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUC-tRNAGln-BbsI-sU6-Kan

Plasmid

#213016

Purpose

Vector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)

Depositor

Stephanie Roessler

Insert

tRNAGln promoter, sU6 promoter, gRNA scaffolds

Use

Tags

Expression

Mutation

Promoter

tRNAGln promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

Plasmid

#25458

Depositor

Betty Eipper

Article

Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

Insert

PAM (Pam Rat)

Use

Tags

His6 and PAM signal

Expression

Mammalian

Mutation

Contains aa 498-820 (PALcc= PAL catalytic core) …

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
SPELL-TEV Component 2

Plasmid

#170490

Purpose

TEV (C-lobe) fused with FRB

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

myc, FRB, TEV(C-lobe)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-TEV Component 1

Plasmid

#170489

Purpose

TEV (N-lobe) fused with iFKBP

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

TEV(N-lobe), iFKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SPELL-GDI Component 2

Plasmid

#170487

Purpose

SPELL GDI1 C-lobe fused to FRB. Split site is 66

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB-GDI1(C-lobe)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-GDI Component 1

Plasmid

#170486

Purpose

SPELL GDI1 N-lobe fused to iFKBP. Split site is 66.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

GDI1(N-lobe)-iFKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-Lyn Component 1

Plasmid

#170484

Purpose

SPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

Lyn(N-lobe), iFKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-Lyn Component 2

Plasmid

#170485

Purpose

SPELL Lyn kinase (Constitutively active) C-lobe fused to FRB. Split site is 279.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Lyn(C-lobe)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-ITSN1(DH-PH) Component 1

Plasmid

#170491

Purpose

SPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

mCherry, ITSN(N-lobe), iFKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-ITSN1(DH-PH) Component 2

Plasmid

#170492

Purpose

SPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

myc, ITSN1(C-lobe), FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

Plasmid

#170488

Purpose

SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge