We narrowed to 86 results for: daniel durocher

1. pCW-eGFP-SHLD3

   Plasmid

   #114126

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDONR-53BP1 1-1972 WT

   Plasmid

   #53446

   Purpose

   Recombinational donor/master vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   siRNA resistant and mutations A128V, T720A, A1347…

   Promoter

   Available since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pDEST-FRT/T0-Flag-BRCA1

   Plasmid

   #71117

   Purpose

   mammalian expression of Flag-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Nov. 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

   Plasmid

   #52504

   Purpose

   mammalian expression vector of BRCA1 wildtype siRNA resistant

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: AGTATAATC

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pcDNA5-FRT/TO-eGFP-SHLD3

   Plasmid

   #114125

   Purpose

   Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNA5-FRT/T0-Flag-53BP1

   Plasmid

   #52507

   Purpose

   mammalian expression vector of 53BP1

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V128A and siRNA resistant sequence: ACGCGCTGACGAC…

   Promoter

   CMV/TO

   Available since

   March 26, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDEST-FRT/TO-3xFLAG-REV7

   Plasmid

   #114127

   Purpose

   Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pDNA5-FRT/TO-eGFP-TONSL

   Plasmid

   #221012

   Purpose

   mammalian expression vector of eGFP tagged TONSL siRNA resistant

   Depositor

   Daniel Durocher

   Article

   O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

   Insert

   TONSL (TONSL Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

   Promoter

   CMV

   Available since

   June 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCW-Flag-CtIP

   Plasmid

   #71108

   Purpose

   mammalian expession of Flag-CtlP

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   TRE promoter, Tet ON

   Available since

   Nov. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA5-FRT/TO-Flag-RNF169

    Plasmid

    #74243

    Purpose

    Expresses RNF169 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

    Insert

    RNF169 (RNF169 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pcDNA3-eGFP-NLS-RNF168

    Plasmid

    #133977

    Purpose

    mammalian expression vector of eGFP tagged wild type RNF168

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pDEST-mCherry-LacR-BRCA1

    Plasmid

    #71115

    Purpose

    mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT/TO-FLAG-SHLD2

    Plasmid

    #114117

    Purpose

    Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

    Plasmid

    #60814

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP-NLS (SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCW-eGFP-FHA-SHLD2

    Plasmid

    #114121

    Purpose

    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    RNF8 FHA domain (aa 2-160) and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pDEST-mCherry-LacR-PALB2

    Plasmid

    #71112

    Purpose

    mammalian expession of mCherry-LacR-PALB2

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

    Plasmid

    #52505

    Purpose

    mammalian expression vector of 53BP1 28A mutant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    53BP1 28A (TP53BP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    All 28 SQ/TQ motifs in N-term of 53BP1 have been …

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMX-HA-UbvG08-IRES-GFP

    Plasmid

    #75030

    Purpose

    Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    Ubiquitin
    

    Use

    Retroviral

    Tags

    HA and IRES-eGFP

    Expression

    Mutation

    UbvG08 with I44A mutation and no terminal Glycines

    Promoter

    Available since

    June 30, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCW-eGFP-FHA-SHLD2-m1

    Plasmid

    #114122

    Purpose

    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (m1 mutant) (SHLD2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    RNF8 FHA domain (aa 2-160) and eGFP

    Expression

    Mammalian

    Mutation

    W489A, F494A, W495A mutations introduced

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pMX-HA-UbvG08_MUT-IRES-GFP

    Plasmid

    #75031

    Purpose

    Retroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant)

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    Ubiquitin
    

    Use

    Retroviral

    Tags

    HA and IRES-eGFP

    Expression

    Mutation

    UbvG08 with I44A, P69L, and L70V mutations and no…

    Promoter

    Available since

    June 30, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge