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Plasmid#89933PurposeKnockdown human Orai1 expressionDepositorInsertOrai1 shRNA (ORAI1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP L273D-L276D
Plasmid#114182PurposeORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.DepositorInsertORAI1-YFP L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationL273D/L276DPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-173-N-ORAI1
Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorInsertVenus173-N-ORAI1 (ORAI1 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-Orai1 E106A pHAGE
Plasmid#22752DepositorInsertOrai1 E106A (ORAI1 Human)
UseLentiviralTagsExpressionMammalianMutationE106APromoterAvailable sinceJan. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
Orai1-myc E106D MO70
Plasmid#22755DepositorInsertOrai1 E106D (ORAI1 Human)
UseRetroviralTagsmycExpressionMammalianMutationE106DPromoterAvailable sinceJan. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
myc-Orai1 E190A pHAGE
Plasmid#22753DepositorInsertOrai1 E190A (ORAI1 Human)
UseLentiviralTagsExpressionMammalianMutationE190APromoterAvailable sinceJan. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M-L273D-L276D
Plasmid#114183PurposeORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationV107M/L273D/L276DPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP T184M-L273D-L276D
Plasmid#114184PurposeORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationT184M/L273D/L276DPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-CAD
Plasmid#73566PurposeSoluble red fluorescent activator of Orai1 channelsDepositorInsertmCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
UseTags6x His and mCherryExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
human STIM1 YFP
Plasmid#19754DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsYFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
human STIM1 CFP
Plasmid#19755DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsCFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hNKD2sg#3
Plasmid#174325PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hNKD2sg#2
Plasmid#174324PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1-Orai3
Plasmid#73563PurposeGreen fluorescent reporter of Orai3-associated calcium influxDepositorInsertG-GECO1-Orai3 (ORAI3 Synthetic, Human)
UseTagsG-GECO1ExpressionMammalianMutationPromoterCMV Immdeiate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-hNKD2
Plasmid#174328Purposeencodes human NKD2 cDNA with a C-terminal GFP-fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-lifeact-mAPPLE
Plasmid#89820PurposemAPPLE labeled fluorescent actinDepositorInsertlifeact-mAPPLE
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN1-mCherry-NKD2
Plasmid#176641PurposeExpresses NKD2-mCherry fusion protein in mammalian cellsDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hNKD2sg#1
Plasmid#174323PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hNKD2
Plasmid#174329Purposeencodes human NKD2 cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsMycHisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only