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Showing: 21 - 40 of 1038 results

  4. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  5. TFORF1000

    Plasmid
    #142251
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF302 (ZNF302 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142251-plasmid-map-sequence-id-454953

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  13. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

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  18. HA-mRIPK3 WT-Flag/cHA-pTRIPZ vector

    Plasmid
    #73701
    Purpose
    Lentiviral expression of mRIPK3
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/tet
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. HA-mRIPK3 K51A-Flag/cHA-pTRIPZ vector

    Plasmid
    #73704
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    K51A
    Promoter
    CMV/tet
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. HA-mRIPK3 D161N-Flag/cHA-pTRIPZ vector

    Plasmid
    #73705
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    D161N
    Promoter
    CMV/tet
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 1038 results