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Plasmid#125443PurposeCRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide3
Plasmid#125442PurposeCRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide2
Plasmid#125441PurposeCRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRE-STOP EF1a-rtTA3
Plasmid#195882Purposecontrol construct of TRE-VEGFA EF1a-rtTA3DepositorInsertVEGFA (Vegfa Mouse, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-6MTnlstdTomato2A
Plasmid#67685PurposeMiddle entry nuclear localized tdTomato reporter with 6xMT and 2ADepositorInserttdTomato
UseTags2A and 6xMyc-NLSExpressionMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmTol-ins-UAS-R1R3-CC
Plasmid#67745PurposeminiTol Destination vector with MAR-flanked UASDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CIRC#del
Plasmid#186344PurposeContains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)DepositorInsertVEGFA (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRC
Plasmid#186343PurposeContains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)DepositorInsertVEGFA (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-SHB
Plasmid#139605PurposeExpresses eGFP-SHB fusion protein in mammalian cellsDepositorInsertSHB (SHB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dusp6DM_S159/197A
Plasmid#67684Purpose3' entry clone with activated Dusp6 (S159/167A)DepositorInsertdusp6 (dusp6 Zebrafish)
UseTagsExpressionMutationS159A, S197APromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR2-mCherry
Plasmid#139606PurposeExpresses a VEGFR2-mCherry fusion protein in mammalian cellsDepositorInsertKDR (KDR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Y1175F-VEGFR2-mCherry
Plasmid#139608PurposeExpresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cellsDepositorInsertKDR (KDR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-1000 to -1)
Plasmid#66128PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -1 bp)DepositorInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -1000 to -1Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Y951F-VEGFR2-mCherry
Plasmid#139607PurposeExpresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cellsDepositorInsertKDR (KDR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Y1214F-VEGFR2-mCherry
Plasmid#139609PurposeExpresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cellsDepositorInsertKDR (KDR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-500 to -1)
Plasmid#66130PurposeLuciferase-based reporter for VEGF promoter region (-500 to -1 bp)DepositorInsertVascular Endothelial Growth Factor-A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -500 to -1Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-1000 to -500)
Plasmid#66129PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -500 bp)DepositorInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -500 to -1Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-950 to -700)
Plasmid#66133PurposeLuciferase-based reporter for VEGF promoter region (-950 to -700 bp)DepositorInsertVascular Endothelial Growth Factor-A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -950 to -700Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
Plasmid#67687PurposeUAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassetteDepositorInsertdusp6 (dusp6 Zebrafish)
UseTags6xmyc-NLS-tdTomato-2AExpressionMutationS159A, S196APromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
5HRE/GFP
Plasmid#46926Purposehypoxia-responsive enhanced green fluorescent protein (EGFP)-based systemDepositorInsert5X HRE of VEGF (VEGFA Human)
UseTagsd2EGFPExpressionMammalianMutationfive copies of a 35-bp fragment from the hypoxia-…PromoterEF-1α promoterAvailable sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only