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pJH4687

Plasmid

#200783

Purpose

Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Use

Tags

pHiuorin

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJW1846

Plasmid

#154326

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP^SEC^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCM1.35

Plasmid

#17248

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

GFP:H2B from pKS111-His in pDONR201

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pWP040

Plasmid

#198777

Purpose

Drives specific expression in the ASG neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PY41C4A.6-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCC12

Plasmid

#198767

Purpose

Drives specific expression in the ADF neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrh-142-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP017

Plasmid

#198780

Purpose

Drives specific expression in the ASI neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pgpa-4-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

Plasmid

#159810

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUA97

Plasmid

#69340

Purpose

It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

Depositor

Oliver Hobert

Insert

ceBFP SEC 3xFlag

Use

Tags

3xFlag and ceBFP (mTagBFP)

Expression

Worm

Mutation

sqt-1(e1350)

Promoter

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1838

Plasmid

#154324

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::CyOFP

Plasmid

#124347

Purpose

Nuclear Localized Pan-Neuronal CyOFP

Depositor

Andrew Leifer

Insert

rab-3, CyOFP (rab-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY2851

Plasmid

#87761

Purpose

Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG(Q103L)

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Punc-17b

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZA_CeTRIC-B1_Del

Plasmid

#87344

Purpose

Inducible expression of CeTRIC-B1 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B1

Use

Tags

polyhistidine

Expression

Yeast

Mutation

Δ248-297

Promoter

AOX1

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Tags

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ353 - MCS(ttTi4391, I)

Plasmid

#34869

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4391 targeting

Use

Worm targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pJH3377

Plasmid

#175286

Purpose

Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16 d/f (daf-16 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Pges-1

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2217

Plasmid

#179251

Purpose

Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Psra-11

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIK127

Plasmid

#65631

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJH2523

Plasmid

#191358

Purpose

Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP3

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Punc-25

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCM1.57

Plasmid

#17252

Depositor

Geraldine Seydoux

Article

Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

Insert

L3788 promoter NO ATG in pDONRP4P1R

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

Plasmid

#159896

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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