We narrowed to 3,042 results for: NAK

1. pAcGFP1-N1-Dsup

   Plasmid

   #90020

   Purpose

   To express GFP-tagged Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK262 (RAD21-mAC Neo)

   Plasmid

   #140538

   Purpose

   RAD21 tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   RAD21 (RAD21 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcGFP1-N1-Dsup-C

   Plasmid

   #90022

   Purpose

   To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   C-terminal region alone

   Promoter

   CMV

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C2-MVP

   Plasmid

   #204543

   Purpose

   Expresses EGFP-tagged MVP in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   MVP (MVP Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

   Plasmid

   #83306

   Purpose

   Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

   Depositor

   Tohru Kimura

   Article

   Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

   Insert

   sgRNA and dCas9 from pX330
   

   Use

   Lentiviral

   Tags

   FLAG and PA tags

   Expression

   Mammalian

   Promoter

   U6 for sgRNA and CBh for dCas9

   Available Since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CTCF-mAC donor (Hygro)

   Plasmid

   #140646

   Purpose

   CTCF tagging with mAID-clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CTCF-mAC donor (Neo)

   Plasmid

   #140645

   Purpose

   CTCF tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHL-H1-ccdB-mEF1a-RiH

   Plasmid

   #60601

   Purpose

   Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   Red Fluorescent Protein
   Hygromycin resistance gene
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHL-EF1a-SphcCas9-iP-A

   Plasmid

   #60599

   Purpose

   Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   CRISPR Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon usage optimized for human usage

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

    Plasmid

    #204547

    Purpose

    Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    deltaUBA

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. DHC1 CRISPR

    Plasmid

    #140545

    Purpose

    DHC1 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX6P-1-MVP

    Plasmid

    #204541

    Purpose

    Expresses GST-tagged MVP in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    MVP (MVP Human)
    

    Expression

    Bacterial

    Mutation

    None

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. DHC1-mIAA17 Hygro

    Plasmid

    #140544

    Purpose

    DHC1 tagging with mIAA7

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

    Plasmid

    #204359

    Purpose

    AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDq-mCherry-P2A-HA-KORD
    

    Use

    AAV

    Tags

    HA (for KORD) and mCherry (for miniDq)

    Mutation

    For miniDq, the third intracellular loop (ICL3) o…

    Promoter

    hSyn

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcGFP1-N1-Dsup-deltaC

    Plasmid

    #90023

    Purpose

    To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal region

    Promoter

    CMV

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXN2KS-Dsup

    Plasmid

    #90019

    Purpose

    To establish stable line expressing Dsup in constitutive manner

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. DHC1-AID-Clover donor (Hygro)

    Plasmid

    #158622

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK297 (DHC1-mAID Hygro)

    Plasmid

    #140542

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-C2-MVP delta607-623aa

    Plasmid

    #204544

    Purpose

    Expresses EGFP-tagged MVP delta607-623aa in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    MVP (MVP Human)
    

    Expression

    Mammalian

    Mutation

    delta607-623aa

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK296 (DHC1-mAID Neo)

    Plasmid

    #140541

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX6P-1-MVP delta607-623aa

    Plasmid

    #204542

    Purpose

    Expresses GST-tagged MVP delta607-623aa in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    MVP (MVP Human)
    

    Expression

    Bacterial

    Mutation

    delta607-623aa

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. DHC1-mIAA7 Neo

    Plasmid

    #140543

    Purpose

    DHC1 tagging with mIAA7

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRetroX-TetOne-Puro-p62

    Plasmid

    #204548

    Purpose

    Expresses p62 in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid

    #80769

    Purpose

    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    IFT88 gRNA#1 (IFT88 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. DHC1-AID-Clover donor (Neo)

    Plasmid

    #158621

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRetroX-TetOne-Puro-p62 deltaUBA

    Plasmid

    #204550

    Purpose

    Expresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    deltaUBA

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. TR01F/hFGFR3-G380R-V5

    Plasmid

    #214908

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR3-G380R variant that is associated with Achondroplasia

    Depositor

    Pavel Krejčí

    Article

    Kimura et al Sci Transl Med. 2021 May 5;13(592):eaba4

    Insert

    FGFR3 (FGFR3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    G380R substitution

    Promoter

    truncated CMV promoter

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRetroX-TetOne-Puro-p62 K7A/D69A

    Plasmid

    #204549

    Purpose

    Expresses p62 K7A/D69A in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    K7A/D69A

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-N-FLAG-HA-TBK1

    Plasmid

    #131791

    Purpose

    Lentiviral vector expressing WT TBK1

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    N-FLAG-HA

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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