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We narrowed to 7,568 results for: Lif

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3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

Plasmid

#60492

Purpose

Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

3xnls-SGFP2(T65G)
SGFP2-3xNES

Use

Tags

SGFP2 and SGFP2(T65G)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-IRES-hrGFP

Plasmid

#107549

Purpose

high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA 5/FR/TO-Ubl4A

Plasmid

#61810

Purpose

express C-terminal Ubl4A, for making the stable cell line

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pRKB134_GI-DUX4-intron2(-)

Plasmid

#83968

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(-) (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

mouseCMV

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pRKB133_GI-DUX4-intron2(+)

Plasmid

#83967

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(+) (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

mouseCMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(chimpanzee)

Plasmid

#79032

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(chimpanzee)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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px330-gp78

Plasmid

#61833

Purpose

to make gp78 Knockout cell line

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

gp78 (AMFR Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR

Plasmid

#67932

Purpose

Translational reporter - Lox 5'UTR

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

sfGFP-NLS-P2A-NLS-mCherry (LOX Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry::Nrv1::vhhGFP4

Plasmid

#163926

Purpose

Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Vkg::mCherry

Plasmid

#163929

Purpose

Expression of GrabFP-ECM under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

G1767D

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJW3.42

Plasmid

#62866

Purpose

Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

PPTR-1/pLexA-N bait

Use

Two-hybrid bait vector

Tags

LexA

Expression

Yeast

Mutation

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 5/FR/TO-Ubl4A K48R

Plasmid

#61811

Purpose

express C-terminal Ubl4A K48R, for making the stable cell line

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K48R

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

Plasmid

#242407

Purpose

Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-moxBFP

Use

Self-amplifying rna

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPRISM-QF2-he1.1:YFP

Plasmid

#184819

Purpose

Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QF2-pA-he1.1:YFP

Use

CRISPR; Backbone for cloning of homology arms (fo…

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-QF2-he1.1:YFP-rln3a-HA

Plasmid

#184820

Purpose

Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

rln3a-HA

Use

CRISPR; For integration of qf2 by homology-direct…

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

Plasmid

#163925

Purpose

Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor

Use

Cre/Lox

Tags

tagBFP

Expression

Insect

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

trc

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)

Plasmid

#79035

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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