We narrowed to 783 results for: Neb

1. pEGFP-RNF8-Middle-part

   Plasmid

   #64672

   Purpose

   Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   ring finger protein 8 (RNF8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Containing only middle part of RNF8 amino acids 1…

   Promoter

   CMV

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMal_Abl-PRM 3R

   Plasmid

   #112086

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   Abl-PRM-3R (ABL1 Human)
   

   Use

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMAL-Abl-PRM 4R

   Plasmid

   #112087

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   Abl-PRM-4R (ABL1 Human)
   

   Use

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMal-Abl-PRM 5R

   Plasmid

   #112088

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   PRM-5R (ABL1 Human)
   

   Use

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CPSF4-1

   Plasmid

   #155478

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CPSF4 (CPSF4 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C

   Plasmid

   #114606

   Purpose

   Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   dNt17 (deletion of first 17 amino acids), Q18C, P…

   Promoter

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C

   Plasmid

   #114602

   Purpose

   Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   dNt17 (deletion of first 17 amino acids), Q18C, P…

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C

   Plasmid

   #114601

   Purpose

   Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   dNt17 (deletion of first 17 amino acids), Q18C, A…

   Promoter

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C

   Plasmid

   #114605

   Purpose

   Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   dNt17 (deletion of first 17 amino acids), Q18C, P…

   Promoter

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C

    Plasmid

    #114604

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, A…

    Promoter

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C

    Plasmid

    #114603

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-22b DT 51E/148K

    Plasmid

    #11081

    Depositor

    John Collier

    Insert

    DT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    51E/148K, catalytically inactive

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRIM3-rfp

    Plasmid

    #74073

    Purpose

    GentR version of pRIM2 carrying rfp

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    rfp
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZ8-Ptac

    Plasmid

    #74064

    Purpose

    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEZ-Frt-lox-DT

    Plasmid

    #11736

    Purpose

    Conditional gene targeting vector; contain cloning sites for 5′ and 3′ homology arms, and a central targeting region flanked by LoxP sites, and a neomycin cassette flanked by FRT sites.

    Depositor

    Klaus Rajewsky

    Insert

    pEZ-Frt-lox-DT
    

    Use

    Cre/Lox; /frt

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZ8-Prp

    Plasmid

    #74065

    Purpose

    pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    Propionate inducible promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prp

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SL-25-0

    Plasmid

    #108673

    Purpose

    in vitro mRNA transcription

    Depositor

    Jon Lorsch

    Article

    Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    CAA repeat mRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SL-150-0

    Plasmid

    #108677

    Purpose

    in vitro mRNA transcription

    Depositor

    Jon Lorsch

    Article

    Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    CAA repeat mRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VanR-wt

    Plasmid

    #153339

    Purpose

    Wild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoter

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAA 659

    Plasmid

    #108684

    Purpose

    in vitro mRNA transcription

    Depositor

    Jon Lorsch

    Article

    Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    CAA repeat mRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge