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Plasmid#201616PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPGM2 (PGM2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-UCK2_sgRNA2
Plasmid#201633PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertUCK2 (UCK2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-RPE_sgRNA2
Plasmid#201619PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertRPE (RPE Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI-UPP2-3xFLAG
Plasmid#201645PurposeOver-expression of 3xFLAG-tagged human UPP2DepositorInsertUPP2 (UPP2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-RPE_sgRNA1
Plasmid#201618PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertRPE (RPE Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-TYMS_sgRNA1
Plasmid#201628PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTYMS (TYMS Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-TYMS_sgRNA2
Plasmid#201629PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTYMS (TYMS Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-UCK1_sgRNA1
Plasmid#201630PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertUCK1 (UCK1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-UCK1_sgRNA2
Plasmid#201631PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertUCK1 (UCK1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-UCK2_sgRNA1
Plasmid#201632PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertUCK2 (UCK2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK10ac-Flag
Plasmid#107427PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertCPK10 (CDPK1 Mustard Weed)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK10-Flag
Plasmid#107428PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertCPK10 (CDPK1 Mustard Weed)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK30ac-Flag
Plasmid#107429PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertCPK30 (CPK30 Mustard Weed)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK30-Flag
Plasmid#107430PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertCPK30 (CPK30 Mustard Weed)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (K24N)
Plasmid#62079PurposeExpression of human p53 (1-73) (K24N) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-73; K24NPromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-2XFLAG-TMEM192
Plasmid#104435PurposeExpresses 2XFLAG-TMEM192 in mammalian cellsDepositorInsertTMEM-192 (TMEM192 Human)
UseLentiviralTags2XFLAGExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 TMEM192-Flag-FKBP
Plasmid#112743Purposeexpress TMEM192-Flag-FKBPDepositorInsertTMEM192-Flag-FKBP (TMEM192 Human)
UseLentiviralTagsFlag, FKBPExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL2(1-380) in pLJM60
Plasmid#184562PurposeCMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertNPRL2 (NPRL2 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines.PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag GFP 4E-BP1 Rheb15
Plasmid#112761Purposeexpress GFP-4EBP1-Rheb15DepositorInsertFlag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
UseLentiviralTagsFlag eGFP and Rheb15ExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 GST MetaP2
Plasmid#112750Purposeexpress HA-GST-FRB-MetaP2DepositorInsertHA-GST-FRB-MetaP2 (METAP2 Human)
UseTagsGSTExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only