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pRK5-mEGFP-MYOD1-MUT

Plasmid

#194571

Purpose

Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MYOD1 (MYOD1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R188Pfs*90

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PICK1-rescue

Plasmid

#72573

Purpose

Expresses myc-PICK1 shRNA resistant cDNA

Depositor

Richard Huganir, Victor Anggono

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Ubiquitin-K27R-K29R

Plasmid

#121156

Purpose

Mammalian expression of HA tagged ubiquitin K27R and K29R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Use

Tags

Expression

Mammalian

Mutation

K27 and K29 mutated to Rs

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-PLBD2-6xHis S392A

Plasmid

#213607

Purpose

Expresses PLBD2-6xHis S392A in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

phospholipase B domain containing 2 (PLBD2 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Changed serine 392 to alanine

Promoter

CMV

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXF1-MUT

Plasmid

#194569

Purpose

Mammalian Expression of mEGFP-FOXF1 Mutant (A231Rfs*61) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXF1 (FOXF1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

A231Rfs*61

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag Grb10 5A short

Plasmid

#37484

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 5A (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform C (missing N-term 58aa), 536aa, S150A T15…

Promoter

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-H1-shRNA-CMV-HA-PACSIN1

Plasmid

#72577

Purpose

Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

Depositor

Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Inserts

PACSIN1 (Pacsin1 Mouse)
H1-PACSIN1 shRNA cassette

Use

RNAi

Tags

HA

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV and H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K840R

Plasmid

#204418

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 840 to Arginine (K840R)

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K1097R

Plasmid

#204417

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 1097 to Arginine (K1097R)

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-rat PKA-RIIbeta

Plasmid

#45522

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 Fyn SH2:R176E

Plasmid

#16035

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

SH2 point mutant: R176E

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pRK5 Flag Grb10 9A long

Plasmid

#37485

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 9A (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform A, 594aa, S104A S150A T155A S158A S426A S…

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-LYCHOS (GPR155) WT-myc

Plasmid

#199684

Purpose

Transient expression of LYCHOS (GPR155) WT

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) 4CA-HA

Plasmid

#199677

Purpose

Transient expression of LYCHOS (GPR155) C595.604.629.638A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C595.604.629.638 mutated to A

Promoter

CMV

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-PSK2 (1-416)

Plasmid

#197123

Purpose

Expression of Myc-tagged PSK2 (aa1-416) / TAOK1 (aa1-416) in mammalian cells

Depositor

Jonathan Morris

Article

Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

Insert

TAOK1 (aa1-416) (TAOK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deleted C-terminus

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 R425V

Plasmid

#47898

Purpose

Expresses myc tagged Miro2 R425V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 R425V (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

R425V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 S430N

Plasmid

#47899

Purpose

Expresses myc tagged Miro2 S430N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 S430N (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S430N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only